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验证数据展示

  • WB analysis using 84494-2-RR

    WB analysis using 84494-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84494-2-RR (EPCR/CD201 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse placenta using 84494-2-RR

    IHC staining of mouse placenta using 84494-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 84494-2-RR (EPCR/CD201 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse placenta using 84494-2-RR

    IHC staining of mouse placenta using 84494-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 84494-2-RR (EPCR/CD201 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse placenta using 84494-2-RR

    IF Staining of mouse placenta using 84494-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse placenta tissue using EPCR/CD201 antibody (84494-2-RR, Clone: 241853A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of bEnd.3 using 84494-2-RR

    IF Staining of bEnd.3 using 84494-2-RR

    Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using EPCR/CD201 antibody (84494-2-RR, Clone: 241853A9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

EPCR/CD201 Recombinant monoclonal antibody

EPCR/CD201 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 84494-2-RR

产品说明书

CloneNo. 241853A9

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

241853A9, Activated protein C receptor, APC receptor, CD201, Centrocyclin

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse lung tissue, mouse placenta tissue
Positive IHC detected inmouse placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse placenta tissue
Positive IF/ICC detected inbEnd.3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84494-2-RR targets EPCR/CD201 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg1517

Product name: Recombinant Mouse EPCR/CD201 protein (rFc Tag)(HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 18-214 aa of NM_011171.2

Sequence: LCNSDGSQSLHMLQISYFQDNHHVRHQGNASLGKLLTHTLEGPSQNVTILQLQPWQDPESWERTESGLQIYLTQFESLVKLVYRERKENVFFPLTVSCSLGCELPEEEEEGSEPHVFFDVAVNGSAFVSFRPKTAVWVSGSQEPSKAANFTLKQLNAYNRTRYELQEFLQDTCVEFLENHITTQNMKGSQTGRSYTS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein C receptor, endothelial
别名 241853A9, Activated protein C receptor, APC receptor, CD201, Centrocyclin
计算分子量27kDa
观测分子量40-50 kDa
GenBank蛋白编号NM_011171.2
基因名称 PROCR
Gene ID (NCBI) 19124
RRIDAB_3672007
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ64695
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein C receptor (PROCR), also known as activated protein C receptor (APC receptor), EPCR, or CD201, is a transmembrane glycoprotein. EPCR promotes the activation of protein C, which has anticoagulant and cytoprotective effects. EPCR facilitates the activation of protein C by the thrombin-thrombomodulin complex but also facilitates APC-mediated cytoprotective impact on cells that involve the activation of protease-activated receptors (PAR) 1 and 3(PMID: 27207424).

实验方案

Product Specific Protocols
IF protocol for EPCR/CD201 antibody 84494-2-RRDownload protocol
WB protocol for EPCR/CD201 antibody 84494-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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