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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 84494-2-RR

    WB analysis using 84494-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84494-2-RR (EPCR/CD201 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse placenta using 84494-2-RR

    IF Staining of mouse placenta using 84494-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse placenta tissue using EPCR/CD201 antibody (84494-2-RR, Clone: 241853A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EPCR/CD201 Recombinant antibody

EPCR/CD201 Recombinant Antibody for WB, IF-P, ELISA
Cat No. 84494-2-RR

产品说明书

CloneNo. 241853A9

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IF-P, ELISA

别名

241853A9, Activated protein C receptor, APC receptor, CD201, Centrocyclin

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse lung tissue, mouse placenta tissue
Positive IF-P detected inmouse placenta tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84494-2-RR targets EPCR/CD201 in WB, IF-P, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 mouse
免疫原 EPCR/CD201 fusion protein Eg1517 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein C receptor, endothelial
别名 241853A9, Activated protein C receptor, APC receptor, CD201, Centrocyclin
计算分子量27kDa
观测分子量40-50 kDa
GenBank蛋白编号NM_011171.2
基因名称 PROCR
Gene ID (NCBI) 19124
RRIDAB_3672007
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ64695
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein C receptor (PROCR), also known as activated protein C receptor (APC receptor), EPCR, or CD201, is a transmembrane glycoprotein. EPCR promotes the activation of protein C, which has anticoagulant and cytoprotective effects. EPCR facilitates the activation of protein C by the thrombin-thrombomodulin complex but also facilitates APC-mediated cytoprotective impact on cells that involve the activation of protease-activated receptors (PAR) 1 and 3(PMID: 27207424).

实验方案

Product Specific Protocols
WB protocol for EPCR/CD201 antibody 84494-2-RRDownload protocol
IF protocol for EPCR/CD201 antibody 84494-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols