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验证数据展示

  • WB analysis using 21688-1-AP

    WB analysis using 21688-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21688-1-AP (PRMT7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 21688-1-AP

    WB analysis using 21688-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21688-1-AP (PRMT7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 21688-1-AP

    IP experiment of HeLa using 21688-1-AP

    IP result of anti-PRMT7 (IP:21688-1-AP, 4ug; Detection:21688-1-AP 1:1000) with HeLa cells lysate 1400 ug.

PRMT7 Polyclonal antibody

PRMT7 Polyclonal Antibody for WB, IP, ELISA
Cat No. 21688-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

别名

Protein arginine N-methyltransferase 7, PRMT 7, Histone-arginine N-methyltransferase PRMT7, EC:2.1.1.321, [Myelin basic protein]-arginine N-methyltransferase PRMT7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells, HeLa cells, MCF-7 cells, MDA-MB-231 cells
Positive IP detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21688-1-AP targets PRMT7 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 PRMT7 fusion protein Ag16269 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein arginine methyltransferase 7
别名 Protein arginine N-methyltransferase 7, PRMT 7, Histone-arginine N-methyltransferase PRMT7, EC:2.1.1.321, [Myelin basic protein]-arginine N-methyltransferase PRMT7
计算分子量 78 kDa
观测分子量73-78 kDa
GenBank蛋白编号BC000146
基因名称 PRMT7
Gene ID (NCBI) 54496
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NVM4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein arginine methyltransferases (PRMTs) are responsible for arginine methylation of histone or nonhistone proteins to regulate diverse biological processes, including cell proliferation, differentiation and senescence. PRMT7 is expressed in both embryonic stem and germ cells and is implicated in maintenance of muscle stem cell function. overexpression of PRMT7 has been associated with cancer metastasis. PRMT7 automethylation at residue R531 was reported to enhance breast cancer metastasis.

实验方案

Product Specific Protocols
WB protocol for PRMT7 antibody 21688-1-APDownload protocol
IP protocol for PRMT7 antibody 21688-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols