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验证数据展示

  • WB analysis using 82750-3-RR (same clone as 82750-3-PBS)

    WB analysis using 82750-3-RR (same clone as 82750-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82750-3-RR (PRMT5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82750-3-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 82750-3-RR (same clone as 82750-3-PBS)

    WB analysis of HepG2 using 82750-3-RR (same clone as 82750-3-PBS)

    WB result of PRMT5 antibody (82750-3-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRMT5 transfected HepG2 cells. This data was developed using the same antibody clone with 82750-3-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 82750-3-RR (same clone as 82750-3-PBS)

    IHC staining of human colon cancer using 82750-3-RR (same clone as 82750-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82750-3-RR (PRMT5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82750-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRMT5 antibody included
  • IHC staining of human colon cancer using 82750-3-RR (same clone as 82750-3-PBS)

    IHC staining of human colon cancer using 82750-3-RR (same clone as 82750-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82750-3-RR (PRMT5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82750-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRMT5 antibody included
  • IF Staining of HepG2 using 82750-3-RR (same clone as 82750-3-PBS)

    IF Staining of HepG2 using 82750-3-RR (same clone as 82750-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRMT5 antibody (82750-3-RR, Clone: 250553F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82750-3-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02769-1

    Cytometric bead array sample test of MP02769-1

    The mean PRMT5 concentration was determined to be 35.0 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load, 27.2 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP02769-1

    Cytometric bead array standard curve of MP02769-1

    Cytometric bead array standard curve of MP02769-1, PRMT5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82750-4-PBS. Detection antibody: 82750-3-PBS. Standard: Ag9736. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 82750-3-RR

    Affinity and Kinetic Characterization of 82750-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82750-3-RR against Human PRMT5 were performed. The affinity constant is 0.604 nM.

  • Featured Product
  • KD/KO验证

PRMT5 Recombinant monoclonal antibody, PBS Only (Detector)

PRMT5 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 82750-3-PBS

产品说明书

CloneNo. 250553F4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

72 kDa ICln-binding protein, EC:2.1.1.320, Histone-arginine N-methyltransferase PRMT5, Jak-binding protein 1, Protein arginine N methyltransferase 5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PRMT5 Ready-To-Use IHC Kit. PRMT5 primary antibody included.

产品信息

82750-3-PBS targets PRMT5 as part of a matched antibody pair:

MP02769-1: 82750-4-PBS capture and 82750-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9736

Product name: Recombinant human PRMT5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 283-637 aa of BC025979

Sequence: YLEYLSQNRPPPNAYELFAKGYEDYLQSPLQPLMDNLESQTYEVFEKDPIKYSQYQQAIYKCLLDRVPEEEKDTNVQVLMVLGAGRGPLVNASLRAAKQADRRIKLYAVEKNPNAVVTLENWQFEEWGSQVTVVSSDMREWVAPEKADIIVSELLGSFADNELSPECLDGAQHFLKDDGVSIPGEYTSFLAPISSSKLYNEVRACREKDRDPEAQFEMPYVVRLHNFHQLSAPQPCFTFSHPNRDPMIDNNRYCTLEFPVEVNTVLHGFAGYFETVLYQDITLSIRPETHSPGMFSWFPILFPIKQPITVREGQTICVRFWRCSNSKKVWYEWAVTAPVCSAIHNPTGRSYTIGL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein arginine methyltransferase 5
别名 72 kDa ICln-binding protein, EC:2.1.1.320, Histone-arginine N-methyltransferase PRMT5, Jak-binding protein 1, Protein arginine N methyltransferase 5
计算分子量 637 aa, 73 kDa
观测分子量67-70 kDa
GenBank蛋白编号BC025979
基因名称 PRMT5
Gene ID (NCBI) 10419
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO14744
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRMT5 is a Type II enzyme that catalyzes arginine monomethylation and symmetric dimethylation (Rme1 and Rme2s). The many targets of PRMT5 include ribosomal proteins, the histone chaperone nucleoplasmin, p53, and histones. PRMT5 is frequently observed in a complex with the cofactor, methylosome protein 50 (MEP50), which is required for PRMT5 activity. PRMT5 is upregulated in several human malignancies, including lymphomas, lung cancer, breast cancer and colorectal cancer.

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