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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 82750-2-RR (same clone as 82750-2-PBS)

    WB analysis using 82750-2-RR (same clone as 82750-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82750-2-RR (PRMT5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82750-2-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 82750-2-RR (same clone as 82750-2-PBS)

    IP experiment of mouse brain using 82750-2-RR (same clone as 82750-2-PBS)

    IP result of anti-PRMT5 (IP:82750-2-RR, 4ug; Detection:82750-2-RR 1:3000) with mouse brain tissue lysate 1720 ug. This data was developed using the same antibody clone with 82750-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82750-2-RR (same clone as 82750-2-PBS)

    IF Staining of HeLa using 82750-2-RR (same clone as 82750-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRMT5 antibody (82750-2-RR, Clone: 250553B5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82750-2-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 82750-2-RR (same clone as 82750-2-PBS)

    IHC staining of human colon cancer using 82750-2-RR (same clone as 82750-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82750-2-RR (PRMT5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82750-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRMT5 antibody included
  • IHC staining of human colon cancer using 82750-2-RR (same clone as 82750-2-PBS)

    IHC staining of human colon cancer using 82750-2-RR (same clone as 82750-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82750-2-RR (PRMT5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82750-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRMT5 antibody included
  • Affinity and Kinetic Characterization of 82750-2-RR

    Affinity and Kinetic Characterization of 82750-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82750-2-RR against Human PRMT5 were performed. The affinity constant is 0.923 nM.

PRMT5 Recombinant antibody, PBS Only

PRMT5 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 82750-2-PBS

产品说明书

CloneNo. 250553B5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

72 kDa ICln-binding protein, EC:2.1.1.320, Histone-arginine N-methyltransferase PRMT5, Jak-binding protein 1, Protein arginine N methyltransferase 5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PRMT5 Ready-To-Use IHC Kit. PRMT5 primary antibody included.

产品信息

82750-2-PBS targets PRMT5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PRMT5 fusion protein Ag9736 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein arginine methyltransferase 5
别名 72 kDa ICln-binding protein, EC:2.1.1.320, Histone-arginine N-methyltransferase PRMT5, Jak-binding protein 1, Protein arginine N methyltransferase 5
计算分子量 637 aa, 73 kDa
观测分子量68 kDa
GenBank蛋白编号BC025979
基因名称 PRMT5
Gene ID (NCBI) 10419
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14744
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRMT5 is a Type II enzyme that catalyzes arginine monomethylation and symmetric dimethylation (Rme1 and Rme2s). The many targets of PRMT5 include ribosomal proteins, the histone chaperone nucleoplasmin, p53, and histones. PRMT5 is frequently observed in a complex with the cofactor, methylosome protein 50 (MEP50), which is required for PRMT5 activity. PRMT5 is upregulated in several human malignancies, including lymphomas, lung cancer, breast cancer and colorectal cancer.