验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, PC-12 cells, NIH/3T3 cells, Raji cells, HeLa cells, HepG2 cells, HL-60 cells |
Planning an IHC experiment? We recommend our IHCeasy PRMT5 Ready-To-Use IHC Kit. PRMT5 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27109-1-AP targets PRMT5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | PRMT5 fusion protein Ag25896 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein arginine methyltransferase 5 |
| 别名 | 72 kDa ICln binding protein, HRMT1L5, IBP72, Jak binding protein 1, JBP1, PRMT5, SKB1, SKB1 homolog, SKB1Hs |
| 计算分子量 | 637 aa, 73 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC025979 |
| 基因名称 | PRMT5 |
| Gene ID (NCBI) | 10419 |
| RRID | AB_2880760 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14744 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PRMT5 antibody 27109-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
