PRMT1 Monoclonal antibody
PRMT1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Rat, Mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1G8E2
验证数据展示
经过测试的应用
Positive WB detected in | U2OS cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
Planning an IHC experiment? We recommend our IHCeasy PRMT1 Ready-To-Use IHC Kit. PRMT1 primary antibody included.
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:100000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68365-1-Ig targets PRMT1 in WB, ELISA applications and shows reactivity with Human, Rat, Mouse samples.
Tested Applications | WB, ELISA |
Tested Reactivity | Human, Rat, Mouse |
Immunogen | PRMT1 fusion protein Ag29063 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | protein arginine methyltransferase 1 |
Synonyms | ANM1, HCP1, HMT2, HRMT1L2, IR1B4, PRMT1 |
Calculated Molecular Weight | 42 kDa |
Observed Molecular Weight | 38~42 kDa |
GenBank Accession Number | BC019268 |
Gene Symbol | PRMT1 |
Gene ID (NCBI) | 3276 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q99873 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRMT1, the major protein arginine methyltransferase in mammals, catalyzes monomethylation and asymmetric dimethylation of arginine side chains in proteins. Human PRMT1 is encoded by the PRMT1 gene located on chromosome 19 (19q13.3) and composed of 12 exons and 11 introns. At the protein level, human PRMT1 shares a high degree of homology with the different members of the PRMT family that is conserved in eukaryotes. Phylogenetic studies based on the methyltransferase domain highlighted that PRMT1 is closely related to PRMT8.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for PRMT1 antibody 68365-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |