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PRKD3 Polyclonal antibody

PRKD3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 12785-1-AP

Print datasheet

Synonyms

EPK2, PKC NU, PKD3, PRKCN, PRKD3, Protein kinase C nu type, protein kinase D3, Protein kinase EPK2

验证数据展示

  • WB analysis using 12785-1-AP

    WB analysis using 12785-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12785-1-AP (PRKD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 12785-1-AP

    IHC staining of human breast cancer using 12785-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12785-1-AP (PRKD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 12785-1-AP

    IHC staining of human breast cancer using 12785-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12785-1-AP (PRKD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HEK-293 using 12785-1-AP

    IP experiment of HEK-293 using 12785-1-AP

    IP result of anti-PRKD3 (IP:12785-1-AP, 4ug; Detection:12785-1-AP 1:300) with HEK-293 cells lysate 2800ug.

经过测试的应用

Positive WB detected inDU 145 cells, HeLa cells, NIH/3T3 cells, C2C12 cells, Raji cells, Ramos cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12785-1-AP targets PRKD3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB, IP, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PRKD3 fusion protein Ag3499 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase D3
Synonyms EPK2, PKC NU, PKD3, PRKCN, PRKD3, Protein kinase C nu type, protein kinase D3, Protein kinase EPK2
Calculated Molecular Weight611aa,68 kDa; 100 kDa; 890aa
Observed Molecular Weight 68 kDa, 100 kDa
GenBank Accession NumberBC030706
Gene Symbol PRKD3
Gene ID (NCBI) 23683
RRIDAB_2877881
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94806
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRKD3 antibody 12785-1-APDownload protocol
IHC protocol for PRKD3 antibody 12785-1-APDownload protocol
IP protocol for PRKD3 antibody 12785-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF,IP

Exp Mol Med

Clusterin negatively modulates mechanical stress-mediated ligamentum flavum hypertrophy through TGF-β1 signaling

Authors - Chunlei Liu
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humanWB

NAR Cancer

Estrogen receptor alpha mutations regulate gene expression and cell growth in breast cancer through microRNAs

Authors - Spencer Arnesen
View Article