验证数据展示
经过测试的应用
| Positive WB detected in | DU 145 cells, HeLa cells, NIH/3T3 cells, C2C12 cells, Raji cells, Ramos cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | DU 145 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12785-1-AP targets PRKD3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag3499 Product name: Recombinant human PRKD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-345 aa of BC030706 Sequence: MSANNSPPSAQKSVLPTAIPAVLPAASPCSSPKTGLSARLSNGSFSAPSLTNSRGSVHTVSFLLQIGLTRESVTIEAQELSLSAVKDLVCSIVYQKFPECGFFGMYDKILLFRHDMNSENILQLITSTDEIHEGDLVEVVLSALATVEDFQIRPHTLYVHSYKAPTFCDYCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRKRRLSNVSLPGPGLSVPRPLQPEYVALPSEESHVHQEPSKRIPSWSGRPIWMEKMVMCRVKVPHTFAVHSYTRPTICQYCKRLLKGLFRQGMQCKDCKFNCHKRCASKVPRDCLGEVTFNGEPSSLGTDTDIPMDID 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein kinase D3 |
| 别名 | EC:2.7.11.13, EPK2, nPKC-nu, PKD3, PRKCN |
| 计算分子量 | 611aa,68 kDa; 100 kDa; 890aa |
| 观测分子量 | 68 kDa, 100 kDa |
| GenBank蛋白编号 | BC030706 |
| 基因名称 | PRKD3 |
| Gene ID (NCBI) | 23683 |
| RRID | AB_2877881 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O94806 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein kinase D 3 (PRKD3), a serine/threonine protein kinase, functions as a crucial regulator across numerous cancer types. High mRNA and protein levels of PRKD3 were reported to be associated with multiple tumor nodules, vascular infiltration, advanced AJCC staging, and poor prognosis in patients with hepatocellular carcinoma (HCC) after liver resection, highlighting the oncogenic function of PRKD3 (PMID: 39678603, 41980898).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PRKD3 antibody 12785-1-AP | Download protocol |
| IHC protocol for PRKD3 antibody 12785-1-AP | Download protocol |
| IP protocol for PRKD3 antibody 12785-1-AP | Download protocol |
| WB protocol for PRKD3 antibody 12785-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Exp Mol Med Clusterin negatively modulates mechanical stress-mediated ligamentum flavum hypertrophy through TGF-β1 signaling | ||
NAR Cancer Estrogen receptor alpha mutations regulate gene expression and cell growth in breast cancer through microRNAs | ||
Cancer Rep (Hoboken) PRKD3 Overexpression May Improve Survival and Suppresses Proliferation in Colorectal Cancer. |
