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验证数据展示

  • WB analysis using 19982-1-AP

    WB analysis using 19982-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19982-1-AP (PRKCQ-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 19982-1-AP

    WB analysis using 19982-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19982-1-AP (PRKCQ-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • FC experiment of K-562 using 19982-1-AP

    FC experiment of K-562 using 19982-1-AP

    1x10^6 K-562 cells were intracellularly stained with 0.2 ug PRKCQ-Specific Polyclonal antibody (19982-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IP experiment of K-562 using 19982-1-AP

    IP experiment of K-562 using 19982-1-AP

    IP result of anti-PRKCQ-Specific (IP:19982-1-AP, 4ug; Detection:19982-1-AP 1:500) with K-562 cells lysate 1600 ug.

PRKCQ-Specific Polyclonal antibody

PRKCQ-Specific Polyclonal Antibody for WB, FC (Intra), IP, ELISA
Cat No. 19982-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), IP, ELISA

别名

PRKCQ, EC:2.7.11.13, nPKC-theta, PRKCT, protein kinase C

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, MOLT-4 cells
Positive IP detected inK-562 cells
Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy PRKCQ Ready-To-Use IHC Kit. PRKCQ primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19982-1-AP targets PRKCQ-Specific in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), IP, ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase C, theta
别名 PRKCQ, EC:2.7.11.13, nPKC-theta, PRKCT, protein kinase C
计算分子量 82 kDa
观测分子量 72-74 kDa
GenBank蛋白编号NM_006257
基因名称 PRKCQ
Gene ID (NCBI) 5588
RRIDAB_3669319
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ04759
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRKCQ, also named as PRKCT and nPKC-theta, belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and PKC subfamily. PRKCQ catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. PRKCQ is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. It is essential for T-cell receptor (TCR)-mediated T-cell activation, but it is dispensable during TCR-dependent thymocyte development. PRKCQ links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. It is required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. This antibody is specific to PRKCQ.

实验方案

Product Specific Protocols
IP protocol for PRKCQ-Specific antibody 19982-1-APDownload protocol
WB protocol for PRKCQ-Specific antibody 19982-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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