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PKC Epsilon Polyclonal antibody

PKC Epsilon Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 20877-1-AP

Print datasheet

Synonyms

PKC epsilon, PKCE, PRKCE, Protein kinase C epsilon type, protein kinase C, epsilon



经过测试的应用

Positive WB detected inmouse brain tissue, SH-SY5Y cells, rat brain tissue
Positive IP detected inSH-SY5Y cells
Positive IHC detected inmouse brain tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, HeLa cells, U2OS cells
Positive FC detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20877-1-AP targets PKC Epsilon in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited ApplicationsIF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, hamster
Immunogen PKC Epsilon fusion protein Ag14976 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase C, epsilon
Synonyms PKC epsilon, PKCE, PRKCE, Protein kinase C epsilon type, protein kinase C, epsilon
Calculated Molecular Weight 737 aa, 84 kDa
Observed Molecular Weight 84 kDa
GenBank Accession NumberBC109033
Gene Symbol PRKCE
Gene ID (NCBI) 5581
RRIDAB_10697812
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02156
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PKC Epsilon antibody 20877-1-APDownload protocol
IHC protocol for PKC Epsilon antibody 20877-1-APDownload protocol
IF protocol for PKC Epsilon antibody 20877-1-APDownload protocol
IP protocol for PKC Epsilon antibody 20877-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Elife

cAMP-EPAC-PKCε-RIM1α signaling regulates presynaptic long-term potentiation and motor learning

Authors - Xin-Tai Wang
mouseWB

Biochem Pharmacol

Thromboxane A2-TP axis promotes adipose tissue macrophages M1 polarization leading to insulin resistance in obesity

Authors - Ruijie Xu
mouseWB

J Cell Mol Med

Protein kinase C is involved in the neuroprotective effect of berberine against intrastriatal injection of quinolinic acid-induced biochemical alteration in mice.

Authors - Peng Liu
WB

Fish Shellfish Immunol

In vivo study of a novel protein kinase C that mediates immunocompetence and catecholamine biosynthesis in hemocytes of Litopenaeus vannamei by using its potential competitive inhibitor, bisindolylmaleimide I.

Authors - Zhong-Wen Chang
humanWB

Mol Biol Cell

MiR-34a regulates blood-tumor barrier function by targeting protein kinase Cɛ.

Authors - Wei Zhao
humanWB

Oxid Med Cell Longev

p66Shc Mediates Mitochondrial Dysfunction Dependent on PKC Activation in Airway Epithelial Cells Induced by Cigarette Smoke.

Authors - Ming Zhang