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验证数据展示

  • WB analysis using 20845-1-AP

    WB analysis using 20845-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20845-1-AP (PRKAR2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 20845-1-AP

    IHC staining of human stomach cancer using 20845-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20845-1-AP (PRKAR2B antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PRKAR2B Polyclonal antibody

PRKAR2B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20845-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, zebrafish

应用

WB, IHC, ELISA

别名

RII BETA, PRKAR2, cAMP-dependent protein kinase type II-beta regulatory subunit

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20845-1-AP targets PRKAR2B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, zebrafish
免疫原

CatNo: Ag11866

Product name: Recombinant human PRKAR2B protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-418 aa of BC075800

Sequence: MSIEIPAGLTELLQGFTVEVLRHQPADLLEFALQHFTRLQQENERKGTARFCHEGRTWGDLGAAAGGGTPSKGVNFAEEPMQSDSEDGEEEEAAPADAGAFNAPVINRFTRRASVCAEAYNPDEEEDDAESRIIHPKTDDQRNRLQEACKDILLFKNLDPEQMSQVLDAMFEKLVKDGEHVIDQGDDGDNFYVIDRGTFDIYVKCDGVGRCVGNYDNRGSFGELALMYNTPRAATITATSPGALWGLDRVTFRRIIVKNNAKKRKMYESFIESLPFLKSLEFSERLKVVDVIGTKVYNDGEQIIAQGDSADSFFIVESGEVKITMKRKGKSEVEENGAVEIARCSRGQYFGELALVTNKPRAASAHAIGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQLVALFGTNMDIVEPTA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase, cAMP-dependent, regulatory, type II, beta
别名 RII BETA, PRKAR2, cAMP-dependent protein kinase type II-beta regulatory subunit
计算分子量 418 aa, 46 kDa
GenBank蛋白编号BC075800
基因名称 PRKAR2B
Gene ID (NCBI) 5577
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31323
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRKAR2B antibody 20845-1-APDownload protocol
IHC protocol for PRKAR2B antibody 20845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols