• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PRKAR1B Polyclonal antibody

PRKAR1B Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 17991-1-AP

Print datasheet

Synonyms

PRKAR1, PRKAR1B

验证数据展示

  • WB analysis of mouse brain using 17991-1-AP

    WB analysis of mouse brain using 17991-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17991-1-AP (PRKAR1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 17991-1-AP

    IHC staining of human breast cancer using 17991-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17991-1-AP (PRKAR1B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 17991-1-AP

    IHC staining of human breast cancer using 17991-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17991-1-AP (PRKAR1B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, Transfected HEK-293 cells, HeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 17991-1-AP is PRKAR1B Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen PRKAR1B fusion protein Ag12426 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase, cAMP-dependent, regulatory, type I, beta
Synonyms PRKAR1, PRKAR1B
Calculated Molecular Weight 381 aa, 43 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC026734
Gene Symbol PRKAR1B
Gene ID (NCBI) 5575
RRIDAB_2878478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31321
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRKAR1B antibody 17991-1-APDownload protocol
IHC protocol for PRKAR1B antibody 17991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols