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验证数据展示

  • WB analysis using 20358-1-AP (same clone as 20358-1-PBS)

    WB analysis using 20358-1-AP (same clone as 20358-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20358-1-AP (PRKAR1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 20358-1-AP (same clone as 20358-1-PBS)

    IHC staining of mouse testis using 20358-1-AP (same clone as 20358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKAR1A antibody included
  • IHC staining of human stomach cancer using 20358-1-AP (same clone as 20358-1-PBS)

    IHC staining of human stomach cancer using 20358-1-AP (same clone as 20358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKAR1A antibody included
  • IHC staining of human stomach cancer using 20358-1-AP (same clone as 20358-1-PBS)

    IHC staining of human stomach cancer using 20358-1-AP (same clone as 20358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKAR1A antibody included
  • IHC staining of rat salivary gland using 20358-1-AP (same clone as 20358-1-PBS)

    IHC staining of rat salivary gland using 20358-1-AP (same clone as 20358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKAR1A antibody included
  • IHC staining of rat salivary gland using 20358-1-AP (same clone as 20358-1-PBS)

    IHC staining of rat salivary gland using 20358-1-AP (same clone as 20358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKAR1A antibody included
  • IHC staining of mouse brain using 20358-1-AP (same clone as 20358-1-PBS)

    IHC staining of mouse brain using 20358-1-AP (same clone as 20358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKAR1A antibody included
  • IHC staining of mouse brain using 20358-1-AP (same clone as 20358-1-PBS)

    IHC staining of mouse brain using 20358-1-AP (same clone as 20358-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKAR1A antibody included
  • IF Staining of HeLa using 20358-1-AP (same clone as 20358-1-PBS)

    IF Staining of HeLa using 20358-1-AP (same clone as 20358-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 20358-1-AP (PRKAR1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 20358-1-AP (same clone as 20358-1-PBS)

    IF Staining of HeLa using 20358-1-AP (same clone as 20358-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRKAR1A antibody (20358-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 20358-1-PBS in a different storage buffer formulation.

PRKAR1A Polyclonal antibody, PBS Only

PRKAR1A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20358-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PPNAD1, PKR1, CNC1, CNC, CAR

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PRKAR1A Ready-To-Use IHC Kit. PRKAR1A primary antibody included.

产品信息

20358-1-PBS targets PRKAR1A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag14216

Product name: Recombinant human PRKAR1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-100 aa of BC036285

Sequence: MESGSTAASEEARSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKAGTRTDSREDEISPPPPNPVVKGRRRRGAI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1)
别名 PPNAD1, PKR1, CNC1, CNC, CAR
计算分子量 381 aa, 43 kDa
观测分子量 43-45 kDa
GenBank蛋白编号BC036285
基因名称 PRKAR1A
Gene ID (NCBI) 5573
RRIDAB_10697659
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10644
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PKA is a heterotetramer inactive kinase composed of two regulatory and two catalytic submits. The regulatory subunits are encoded by four genes (PRKAR1A, PRKAR2A, PRKAR1B, and PRKAR2B). PRKAR1A, a gene coding for the cAMP-dependent protein kinase (PKA) 1α regulatory submit, is located on human chromosome 17q22-24. PRKAR1A protein insufficiency and PKA dysregulation have been implicated in various types of disorders, including Albright hereditary osteodystrophy (AHO), pseudohypoparathyroidism (PHP), acrodysostosis (ACRDYS), and Carney complex. PRKAR1A protein expression level was significantly dysregulated in multiple primary carcinomas and distant metastases, such as cardiac myxomas, odontogenic myxomas, anaplastic thyroid carcinomas, breast cancer, pediatric pituitary adenomas, and Schwann cell tumors.

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