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验证数据展示

  • WB analysis of HeLa using 67491-1-Ig (same clone as 67491-1-PBS)

    WB analysis of HeLa using 67491-1-Ig (same clone as 67491-1-PBS)

    WB result of PRKACA antibody (67491-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRKACA transfected HeLa cells. This data was developed using the same antibody clone with 67491-1-PBS in a different storage buffer formulation.

  • WB analysis using 67491-1-Ig (same clone as 67491-1-PBS)

    WB analysis using 67491-1-Ig (same clone as 67491-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67491-1-Ig (PRKACA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67491-1-PBS in a different storage buffer formulation.

  • WB analysis using 67491-1-Ig (same clone as 67491-1-PBS)

    WB analysis using 67491-1-Ig (same clone as 67491-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67491-1-Ig (PRKACA antibody) at dilution of 1:2300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67491-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67491-1-Ig (same clone as 67491-1-PBS)

    IHC staining of human breast cancer using 67491-1-Ig (same clone as 67491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67491-1-Ig (PRKACA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67491-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKACA antibody included
  • IHC staining of human breast cancer using 67491-1-Ig (same clone as 67491-1-PBS)

    IHC staining of human breast cancer using 67491-1-Ig (same clone as 67491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67491-1-Ig (PRKACA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67491-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKACA antibody included
  • Featured Product
  • KD/KO验证

PRKACA Monoclonal antibody, PBS Only

PRKACA Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67491-1-PBS

产品说明书

CloneNo. 4G10D7

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB, IHC, ELISA

别名

4G10D7, cAMP-dependent protein kinase catalytic subunit alpha, EC:2.7.11.11, PKA C alpha, PKA C-alpha

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PRKACA Ready-To-Use IHC Kit. PRKACA primary antibody included.

产品信息

67491-1-PBS targets PRKACA in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag26400

Product name: Recombinant human PRKACA protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 162-351 aa of BC039846

Sequence: DLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFSEF

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 protein kinase, cAMP-dependent, catalytic, alpha
别名 4G10D7, cAMP-dependent protein kinase catalytic subunit alpha, EC:2.7.11.11, PKA C alpha, PKA C-alpha
计算分子量 41 kDa
观测分子量 38-43 kDa
GenBank蛋白编号BC039846
基因名称 PRKACA
Gene ID (NCBI) 5566
RRIDAB_2882716
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP17612
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRKACA, also named as PKACA and PKA C-alpha, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It phosphorylates a large number of substrates in the cytoplasm and the nucleus.

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