验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue, human liver tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy PRKACA Ready-To-Use IHC Kit. PRKACA primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55388-1-AP targets PKA C-alpha specific in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, sheep |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein kinase, cAMP-dependent, catalytic, alpha |
| 别名 | PKA C alpha, PKACA, PRKACA |
| 计算分子量 | 41 kDa |
| 观测分子量 | 38-43 kDa |
| GenBank蛋白编号 | NM_002730 |
| 基因名称 | PRKACA |
| Gene ID (NCBI) | 5566 |
| RRID | AB_10950890 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P17612 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PRKACA, also named as PKACA and PKA C-alpha, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It phosphorylates a large number of substrates in the cytoplasm and the nucleus. This antibody is specific to PRKACA.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PKA C-alpha specific antibody 55388-1-AP | Download protocol |
| IP protocol for PKA C-alpha specific antibody 55388-1-AP | Download protocol |
| WB protocol for PKA C-alpha specific antibody 55388-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Total Environ Prolonged exposure of environmental concentration benzo[a]pyrene promoted cancer stemness through AhR/PKA/SOX2 dependent pathway in small cell lung cancer | ||
Int J Mol Sci Gastrointestinal Dopamine in Inflammatory Bowel Diseases: A Systematic Review. | ||
Oncotarget Multi-chaperone function modulation and association with cytoskeletal proteins are key features of the function of AIP in the pituitary gland. | ||
Front Pharmacol An Integrative Pharmacology-Based Strategy to Uncover the Mechanism of Xiong-Pi-Fang in Treating Coronary Heart Disease with Depression. | ||
Phytother Res Rapid antidepressant-like effect of Fructus Aurantii depends on cAMP-response element binding protein/Brain-derived neurotrophic facto by mediating synaptic transmission. | ||
Proteomics Label-Free Quantitative Phosphoproteomics Reveals Regulation of Vasodilator-Stimulated Phosphoprotein Upon Stathmin-1 Silencing in a Pair of Isogenic Colorectal Cancer Cell Lines. |
