• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

PRKACA Polyclonal antibody

PRKACA Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 24503-1-AP

Print datasheet

Synonyms

PKA C alpha, PKACA, PRKACA

验证数据展示

  • WB analysis using 24503-1-AP

    WB analysis using 24503-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24503-1-AP (PRKACA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 24503-1-AP

    WB analysis of HeLa using 24503-1-AP

    WB result of PRKACA antibody (24503-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRKACA transfected HeLa cells.

  • IHC staining of human breast cancer using 24503-1-AP

    IHC staining of human breast cancer using 24503-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24503-1-AP (PRKACA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRKACA antibody included
  • IHC staining of human breast cancer using 24503-1-AP

    IHC staining of human breast cancer using 24503-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24503-1-AP (PRKACA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRKACA antibody included

经过测试的应用

Positive WB detected inNeuro-2a cells, HeLa cells, SH-SY5Y cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PRKACA Ready-To-Use IHC Kit. PRKACA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 24503-1-AP is PRKACA Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen PRKACA fusion protein Ag21478 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase, cAMP-dependent, catalytic, alpha
Synonyms PKA C alpha, PKACA, PRKACA
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 38-43 kDa
GenBank Accession NumberBC039846
Gene Symbol PRKACA
Gene ID (NCBI) 5566
RRIDAB_2879578
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17612
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRKACA antibody 24503-1-APDownload protocol
IHC protocol for PRKACA antibody 24503-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Front Pharmacol

Integrating network pharmacology and experimental verification to explore the mechanism of puerarin against oliguria in acute alcoholism

Authors - Mei-Xuan Wan
View Article