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验证数据展示

  • WB analysis using 14429-1-AP

    WB analysis using 14429-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14429-1-AP (AMPK beta 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 14429-1-AP

    IHC staining of human colon using 14429-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14429-1-AP (AMPK Beta 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRKAB2 antibody included
  • IHC staining of human colon using 14429-1-AP

    IHC staining of human colon using 14429-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14429-1-AP (AMPK Beta 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRKAB2 antibody included
  • IF Staining of HeLa using 14429-1-AP

    IF Staining of HeLa using 14429-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AMPK Beta 2 antibody (14429-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

AMPK Beta 2 Polyclonal antibody

AMPK Beta 2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14429-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

PRKAB2, 5'-AMP-activated protein kinase subunit beta-2, AMPK, AMPK subunit beta 2, AMPK subunit beta-2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, human heart tissue, human brain tissue, fetal human brain tissue, mouse testis tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PRKAB2 Ready-To-Use IHC Kit. PRKAB2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14429-1-AP targets AMPK Beta 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5806

Product name: Recombinant human PRKAB2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-272 aa of BC053610

Sequence: MGNTTSDRVSGERHGAKAARSEGAGGHAPGKEHKIMVGSTDDPSVFSLPDSKLPGDKEFVSWQQDLEDSVKPTQQARPTVIRWSEGGKEVFISGSFNNWSTKIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTINNLIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYAFRSEERFKSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLNHLYALSIKDSVMVLSATHRYKKKYVTTLLYKPI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase, AMP-activated, beta 2 non-catalytic subunit
别名 PRKAB2, 5'-AMP-activated protein kinase subunit beta-2, AMPK, AMPK subunit beta 2, AMPK subunit beta-2
计算分子量 30 kDa
观测分子量 34 kDa
GenBank蛋白编号BC053610
基因名称 AMPK Beta 2
Gene ID (NCBI) 5565
RRIDAB_2252925
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43741
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AMPK beta 2 is a regulatory subunit of the AMP-activated protein kinase (AMPK), which is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK beta 2 may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles.

实验方案

Product Specific Protocols
WB protocol for AMPK Beta 2 antibody 14429-1-APDownload protocol
IHC protocol for AMPK Beta 2 antibody 14429-1-APDownload protocol
IF protocol for AMPK Beta 2 antibody 14429-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols