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验证数据展示

  • WB analysis of MDA-MB-231 using 84926-1-RR

    WB analysis of MDA-MB-231 using 84926-1-RR

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 84926-1-RR (PRICKLE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 84926-1-RR

    IHC staining of mouse cerebellum using 84926-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84926-1-RR (PRICKLE1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PRICKLE1 antibody included
  • Affinity and Kinetic Characterization of 84926-1-RR

    Affinity and Kinetic Characterization of 84926-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84926-1-RR against Human PRICKLE1 were performed. The affinity constant is 7.50 nM.

PRICKLE1 Recombinant antibody

PRICKLE1 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84926-1-RR

产品说明书

CloneNo. 242506A8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

242506A8, EPM1B, Prickle-like protein 1, REST/NRSF-interacting LIM domain protein 1, RILP

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMDA-MB-231 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PRICKLE1 Ready-To-Use IHC Kit. PRICKLE1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84926-1-RR targets PRICKLE1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 PRICKLE1 fusion protein Ag18407 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 prickle homolog 1 (Drosophila)
别名 242506A8, EPM1B, Prickle-like protein 1, REST/NRSF-interacting LIM domain protein 1, RILP
计算分子量 831 aa, 94 kDa
观测分子量 94 kDa
GenBank蛋白编号BC114939
基因名称 PRICKLE1
Gene ID (NCBI) 144165
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96MT3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRICKLE1, also known as RILP, EPM1B, encodes a cytoplasmic protein with an N-terminal PET (Pk, Espinas, Testin) domain, three LIM domains and a C-terminal PKH (prickle homologous) domain. PRICKLE1 is expressed at the highest levels in placenta and lower levels in lung, liver, kidney and pancreas(PMID: 12525887). PRICKLE1 localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in PRICKLE1 have been linked to progressive myoclonus epilepsy(PMID: 18976727).

实验方案

Product Specific Protocols
WB protocol for PRICKLE1 antibody 84926-1-RRDownload protocol
IHC protocol for PRICKLE1 antibody 84926-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols