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验证数据展示

  • WB analysis of HepG2 using 21460-1-AP

    WB analysis of HepG2 using 21460-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21460-1-AP (PRH1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • IF Staining of HepG2 using 21460-1-AP

    IF Staining of HepG2 using 21460-1-AP

    Immunofluorescent analysis of HepG2 cells using 21460-1-AP (PRH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

PRH1 Polyclonal antibody

PRH1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 21460-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

PRPC, Peptide P-C, Parotid proline-rich protein 1/2, Parotid double-band protein, Pa

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21460-1-AP targets PRH1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14833

Product name: Recombinant human PRH1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 17-187 aa of BC064553

Sequence: QDLNEDVSQEDVPLVISDGGDSEQFLDEERQGPPLGGQQSQPSAGDGNQDDGPQQGPPQQGGQQQQGPPPPQGKPQGPPQQGGQQQQGPPPPQGKPQGPPQQGGHPPPPQGRPQGPPQQGGHPRPPRGRPQGPPQQGGHQQGPPPPPPGKPQGPPPQGGRPQGPPQGQSPQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proline-rich protein HaeIII subfamily 1
别名 PRPC, Peptide P-C, Parotid proline-rich protein 1/2, Parotid double-band protein, Pa
计算分子量 17 kDa
观测分子量 17 kDa
GenBank蛋白编号BC064553
基因名称 PRH1
Gene ID (NCBI) 5554
RRIDAB_2878862
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP02810
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRH1, also known as Salivary acidic proline-rich phosphoprotein 1/2, is a 166 amino acid secreted protein whose molecular weight is 17 kDa. It functions as a highly potent inhibitor of calcium phosphate crystal growth. Similar to other PRPs, PRH1 provides a protective and reparative environment for dental enamel, which is important for teeth integrity. This antibody specifically recognises the 17 kDa protein.

实验方案

Product Specific Protocols
IF protocol for PRH1 antibody 21460-1-APDownload protocol
WB protocol for PRH1 antibody 21460-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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