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PREX2 Polyclonal antibody

PREX2 Polyclonal Antibody for IF/ICC, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC,ELISA

Conjugate

Unconjugated

Cat No : 24195-1-AP

Print datasheet

Synonyms

DEP.2, DEPDC2, P REX2, PREX2

验证数据展示

  • IHC staining of human heart using 24195-1-AP

    IHC staining of human heart using 24195-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24195-1-AP (PREX2 antibody) at dilution of 1:200 (under 10x lens)..

  • IHC staining of human heart using 24195-1-AP

    IHC staining of human heart using 24195-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24195-1-AP (PREX2 antibody) at dilution of 1:200 (under 40x lens)..

  • IF Staining of U2OS using 24195-1-AP

    IF Staining of U2OS using 24195-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 24195-1-AP (PREX2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman heart tissue, human cerebellum tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24195-1-AP is PREX2 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC,ELISA
Tested Reactivity human
Immunogen PREX2 fusion protein Ag19439 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2
Synonyms DEP.2, DEPDC2, P REX2, PREX2
Calculated Molecular Weight 1606 aa, 183 kDa
Observed Molecular Weight 182 kDa
GenBank Accession NumberBC148795
Gene Symbol PREX2
Gene ID (NCBI) 80243
RRIDAB_2879450
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ70Z35
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for PREX2 antibody 24195-1-APDownload protocol
IF protocol for PREX2 antibody 24195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols