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验证数据展示

  • IHC staining of human heart using 24195-1-AP

    IHC staining of human heart using 24195-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24195-1-AP (PREX2 antibody) at dilution of 1:200 (under 10x lens)..

  • IHC staining of human heart using 24195-1-AP

    IHC staining of human heart using 24195-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24195-1-AP (PREX2 antibody) at dilution of 1:200 (under 40x lens)..

  • IF Staining of U2OS using 24195-1-AP

    IF Staining of U2OS using 24195-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 24195-1-AP (PREX2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

PREX2 Polyclonal antibody

PREX2 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 24195-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

P-Rex2, Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein, P REX2, DEPDC2, DEP.2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman heart tissue, human cerebellum tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24195-1-AP targets PREX2 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19439

Product name: Recombinant human PREX2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 648-979 aa of BC148795

Sequence: PFNEVDCFLKSCLNSRKPLRVLVSTKPRETVKIPDSADGLGFQIRGFGPSVVHAVGRGTVAAAAGLHPGQCIIKVNGINVSKETHASVIAHVTACRKYRRPTKQDSIQWVYNSIESAQEDLQKSHSKPPGDEAGDAFDCKVEEVIDKFNTMAIIDGKKEHVSLTVDNVHLEYGVVYEYDSTAGIKCNVVEKMIEPKGFFSLTAKILEALAKSDEHFVQNCTSLNSLNEVIPTDLQSKFSALCSERIEHLCQRISSYKKVQASERFYNFTARHAVWEHSFDLHSVSSTFPVPVTMEFLLLPPPLLGISQDGRQHCIPEDLPSQEMLLAERAPV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2
别名 P-Rex2, Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein, P REX2, DEPDC2, DEP.2
计算分子量 1606 aa, 183 kDa
观测分子量 182 kDa
GenBank蛋白编号BC148795
基因名称 PREX2
Gene ID (NCBI) 80243
RRIDAB_2879450
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ70Z35
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for PREX2 antibody 24195-1-APDownload protocol
IHC protocol for PREX2 antibody 24195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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