• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 33193-1-AP

    WB analysis using 33193-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33193-1-AP (PREX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 33193-1-AP

    IF Staining of MCF-7 using 33193-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PREX1 antibody (33193-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PREX1 Polyclonal antibody

PREX1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 33193-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

KIAA1415, P REX1, Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein, P-Rex1, PtdIns(3,4,5)-dependent Rac exchanger 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inMCF-7 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33193-1-AP targets PREX1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag36649

Product name: Recombinant human PREX1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 700-900 aa of NM_020820.3

Sequence: LLVATKAKEIIKIPDQPDTLCFQIRGAAPPYVYAVGRGSEAMAAGLCAGQCILKVNGSNVMNDGAPEVLEHFQAFRSRREEALGLYQWIYHTHEDAQEARASQEASTEDPSGEQAQEEDQADSAFPLLSLGPRLSLCEDSPMVTLTVDNVHLEHGVVYEYVSTAGVRCHVLEKIVEPRGCFGLTAKILEAFAANDSVFVEN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 1
别名 KIAA1415, P REX1, Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein, P-Rex1, PtdIns(3,4,5)-dependent Rac exchanger 1
计算分子量186 kDa
观测分子量186 kDa
GenBank蛋白编号NM_020820.3
基因名称 PREX1
Gene ID (NCBI) 57580
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8TCU6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (PREX1) is a guanine nucleotide exchange factor (GEF) that specifically activates Rac GTPases, particularly Rac1 (PMID: 16243035). PREX1 is activated downstream of G protein-coupled receptors (GPCRs) and receptor tyrosine kinases (RTKs), such as ErbB receptors, and is involved in PI3K/AKT and MAPK signaling pathways (PMID: 28710285; 27358402). It contributes to glucose uptake in adipocytes and promotes tumor cell proliferation, migration, and metastasis in various cancers, including breast, prostate, and melanoma (PMID: 22109529).

实验方案

Product Specific Protocols
IF protocol for PREX1 antibody 33193-1-APDownload protocol
WB protocol for PREX1 antibody 33193-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...