验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human brain tissue |
| Positive IHC detected in | rat brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12478-1-AP targets PREPL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag3196 Product name: Recombinant human PREPL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-244 aa of BC013193 Sequence: MDLKMNFRPERRVLVDDGWILAYCHVRYGGELGLQWHADGRLTKKLNGLADLEACIKTLHGQGFSQPSLTTLTAFSAGGVLAGALCNSNPELVRAVTLEAPFLDVLNTMMDTTLPLTLEELEEWGNPSSDEKHKNYIKRYCPYQNIKPQHYPSIHITAYENDERVPLKGIVSYTEKLKEAIAEHAKDTGEGYQTPNIILDIQPGGNHVIEDSHKKITAQIKFLYEELGLDSTSVFEDLKKYLKF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | prolyl endopeptidase-like |
| 别名 | EC:3.4.21.-, KIAA0436, PPCEL, prolyl endopeptidase like, Prolyl endopeptidase-like |
| 计算分子量 | 72 kDa |
| 观测分子量 | 72 kDa |
| GenBank蛋白编号 | BC013193 |
| 基因名称 | PREPL |
| Gene ID (NCBI) | 9581 |
| RRID | AB_2300040 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q4J6C6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PREPL (Prolyl endopeptidase-like) is also named as KIAA0436 and belongs to the prolyl oligopeptidase subfamily of serine peptidases. IPREPL is a novel oligopeptidase with unique structural and functional characteristics (PMID:16385448). It has 4 isoforms produced by alternative splicing and can exsit as a homodimer (PMID:16143824). Defects in PREPL are a cause of hypotonia-cystinuria syndrome (HCS).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PREPL antibody 12478-1-AP | Download protocol |
| WB protocol for PREPL antibody 12478-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
