×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis of human lung using 11536-1-AP (same clone as 11536-1-PBS)

    WB analysis of human lung using 11536-1-AP (same clone as 11536-1-PBS)

    human lung tissue were subjected to SDS PAGE followed by western blot with 11536-1-AP (PREP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 11536-1-AP (same clone as 11536-1-PBS)

    IHC staining of human ovary tumor using 11536-1-AP (same clone as 11536-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11536-1-AP (PREP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 11536-1-AP (same clone as 11536-1-PBS)

    IHC staining of human ovary tumor using 11536-1-AP (same clone as 11536-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11536-1-AP (PREP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 11536-1-AP (same clone as 11536-1-PBS)

    IF Staining of A549 using 11536-1-AP (same clone as 11536-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 11536-1-AP (PREP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11536-1-AP (same clone as 11536-1-PBS)

    IHC staining of human colon cancer using 11536-1-AP (same clone as 11536-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11536-1-AP (PREP antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11536-1-AP (same clone as 11536-1-PBS)

    IHC staining of human colon cancer using 11536-1-AP (same clone as 11536-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11536-1-AP (PREP antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11536-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PREP Polyclonal antibody, PBS Only

PREP Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11536-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Post-proline cleaving enzyme, PEP, EC:3.4.21.26

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

11536-1-PBS targets PREP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2101

Product name: Recombinant human PREP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 360-710 aa of BC030636

Sequence: ILQLHDLTTGALLKTFPLDVGSIVGYSGQKKDTEIFYQFTSFLSPGIIYHCDLTKEELEPRVFREVTVKGIDASDYQTVQIFYPSKDGTKIPMFIVHKKGIKLDGSHPAFLYGYGGFNISITPNYSVSRLIFVRHMGGILAVANIRGGGEYGETWHKGGILANKQNCFDDFQCAAEYLIKEGYTSPKRLTINGGSNGGLLVAACANQRPDLFGCVIAQVGVMDMLKFHKYTIGHAWTTDYGCSDSKQHFEWLVKYSPLHNVKLPEADDIQYPSMLLLTADHDDRVVPLHSLKFIATLQYIVGRSRKQSNPLLIHVDTKAGHGAGKPTAKVIEEVSDMFAFIARCLNVDWIP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 prolyl endopeptidase
别名 Post-proline cleaving enzyme, PEP, EC:3.4.21.26
计算分子量 710 aa, 81 kDa
观测分子量 81 kDa
GenBank蛋白编号BC030636
基因名称 PREP
Gene ID (NCBI) 5550
RRIDAB_2284249
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48147
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PREP, also named as PE and PEP, belongs to the peptidase S9A family. It cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Together with MMPs, PREP may play an important role in the formation of PGP and thus in the pathophysiology of lung emphysema.PREP is the only enzyme directly capable of cleaving PGP from shorter portions of collagen.(PMID:21112944)

||
New chat

Able™

正在加载，请稍候...