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验证数据展示

  • WB analysis of Daudi using 66981-1-Ig (same clone as 66981-1-PBS)

    WB analysis of Daudi using 66981-1-Ig (same clone as 66981-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 66981-1-Ig (PREB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66981-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66981-1-Ig (same clone as 66981-1-PBS)

    IHC staining of human prostate cancer using 66981-1-Ig (same clone as 66981-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66981-1-Ig (PREB antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66981-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PREB antibody included

PREB Monoclonal antibody, PBS Only

PREB Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66981-1-PBS

产品说明书

CloneNo. 2F6A1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

2F6A1, Guanine nucleotide-exchange factor SEC12, Prolactin regulatory element-binding protein, SEC12

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PREB Ready-To-Use IHC Kit. PREB primary antibody included.

产品信息

66981-1-PBS targets PREB in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag28549

Product name: Recombinant human PREB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 13-202 aa of BC002765

Sequence: PFPLYALQVDPSTGLLIAAGGGGAAKTGIKNGVHFLQLELINGRLSASLLHSHDTETRATMNLALAGDILAAGQDAHCQLLRFQAHQQQGNKAEKAGSKEQGPRQRKGAAPAEKKCGAETQHEGLELRVENLQAVQTDFSSDPLQKVVCFNHDNTLLATGGTDGYVRVWKVPSLEKVLEFKAHEGEIEDL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 prolactin regulatory element binding
别名 2F6A1, Guanine nucleotide-exchange factor SEC12, Prolactin regulatory element-binding protein, SEC12
计算分子量 45 kDa
观测分子量 45 kDa
GenBank蛋白编号BC002765
基因名称 PREB
Gene ID (NCBI) 10113
RRIDAB_2882301
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9HCU5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PREB contains two PQ-rich potential transactivation domains, but no apparent DNA-binding motif, and exhibits sequence-specific binding to Pit1-binding element of the prolactin (PRL) promoter. It may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p.

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