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- KD/KO Validated
PRDX1 Polyclonal antibody
PRDX1 Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC, IF/ICC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HEK-293 cells, U2OS cells, HeLa cells, HepG2 cells, mouse kidney tissue, rat kidney tissue |
Positive IHC detected in | human breast cancer tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells, RAW 264.7 cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15816-1-AP targets PRDX1 in WB, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IF, IHC, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | PRDX1 fusion protein Ag8602 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | peroxiredoxin 1 |
Synonyms | MSP23, NKEF A, NKEFA, PAG, PAGA, PAGB, peroxiredoxin 1, PRDX1, PRX1, PRXI, TDPX2, Thioredoxin peroxidase 2 |
Calculated Molecular Weight | 199 aa, 22 kDa |
Observed Molecular Weight | 23 kDa |
GenBank Accession Number | BC007063 |
Gene Symbol | PRDX1 |
Gene ID (NCBI) | 5052 |
RRID | AB_2170318 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q06830 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for PRDX1 antibody 15816-1-AP | Download protocol |
IHC protocol for PRDX1 antibody 15816-1-AP | Download protocol |
IF protocol for PRDX1 antibody 15816-1-AP | Download protocol |
FC protocol for PRDX1 antibody 15816-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance. | ||
Br J Pharmacol Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation. | ||
Cell Chem Biol Parvifoline AA Promotes Susceptibility of Hepatocarcinoma to Natural Killer Cell-Mediated Cytolysis by Targeting Peroxiredoxin. | ||
Cell Rep The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense. | ||
Front Immunol A novel cuproptosis pattern and tumor immune microenvironment characterization in urothelial carcinoma of the bladder |