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PRDM5 Polyclonal antibody

PRDM5 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 14126-1-AP

Print datasheet

Synonyms

PFM2, PR domain containing 5, PR domain containing protein 5, PRDM5

验证数据展示

  • WB analysis of mouse ovary using 14126-1-AP

    WB analysis of mouse ovary using 14126-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14126-1-AP (PRDM5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 14126-1-AP

    IHC staining of human colon cancer using 14126-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14126-1-AP (PRDM5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse ovary tissue, mouse colon tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14126-1-AP targets PRDM5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen PRDM5 fusion protein Ag5277 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PR domain containing 5
Synonyms PFM2, PR domain containing 5, PR domain containing protein 5, PRDM5
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC066942
Gene Symbol PRDM5
Gene ID (NCBI) 11107
RRIDAB_2878019
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQX1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRDM5 antibody 14126-1-APDownload protocol
IHC protocol for PRDM5 antibody 14126-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols