• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IHC staining of human colon cancer using 14126-1-AP

    IHC staining of human colon cancer using 14126-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14126-1-AP (PRDM5 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse ovary using 14126-1-AP

    WB analysis of mouse ovary using 14126-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14126-1-AP (PRDM5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

PRDM5 Polyclonal antibody

PRDM5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14126-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

PR domain-containing protein 5, PR domain containing protein 5, PR domain containing 5, PFM2, EC:2.1.1.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse ovary tissue, mouse colon tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14126-1-AP targets PRDM5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5277

Product name: Recombinant human PRDM5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-111 aa of BC066942

Sequence: MLGMYVPDRFSLKSSRVQDGMGLYTARRVRKGEKFGPFAGEKRMPEDLDENMDYRLMWEVRGSKGEVLYILDATNPRHSNWLRFVHEAPSQEQKNLAAIQDKNLGPAEWRG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PR domain containing 5
别名 PR domain-containing protein 5, PR domain containing protein 5, PR domain containing 5, PFM2, EC:2.1.1.-
计算分子量 73 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC066942
基因名称 PRDM5
Gene ID (NCBI) 11107
RRIDAB_2878019
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQX1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PRDM5 antibody 14126-1-APDownload protocol
IHC protocol for PRDM5 antibody 14126-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols