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验证数据展示

  • WB analysis using 29653-1-AP (same clone as 29653-1-PBS)

    WB analysis using 29653-1-AP (same clone as 29653-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29653-1-AP (PPT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29653-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 29653-1-AP (same clone as 29653-1-PBS)

    IHC staining of human kidney using 29653-1-AP (same clone as 29653-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29653-1-AP (PPT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29653-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 29653-1-AP (same clone as 29653-1-PBS)

    IHC staining of human kidney using 29653-1-AP (same clone as 29653-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29653-1-AP (PPT1 antibody) at dilution of 1:400 (under 10x lens). Heat-mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29653-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 29653-1-AP (same clone as 29653-1-PBS)

    IF Staining of mouse testis using 29653-1-AP (same clone as 29653-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PPT1 antibody (29653-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29653-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 29653-1-AP (same clone as 29653-1-PBS)

    WB analysis of A549 using 29653-1-AP (same clone as 29653-1-PBS)

    WB result of PPT1 antibody (29653-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPT1 transfected A549 cells. This data was developed using the same antibody clone with 29653-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 29653-1-AP (same clone as 29653-1-PBS)

    IF Staining of rat brain using 29653-1-AP (same clone as 29653-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using PPT1 antibody (29653-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 29653-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PPT1 Polyclonal antibody, PBS Only

PPT1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 29653-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

CLN1, EC:3.1.2.22, INCL, Palmitoyl-protein hydrolase 1, Palmitoyl-protein thioesterase 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

29653-1-PBS targets PPT1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag31240

Product name: Recombinant human PPT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 150-306 aa of BC008426

Sequence: PRCPGESSHICDFIRKTLNAGAYSKVVQERLVQAEYWHDPIKEDVYRNHSIFLADINQERGINESYKKNLMALKKFVMVKFLNDSIVDPVDSEWFGFYRSGQAKETIPLQETSLYTQDRLGLKEMDNAGQLVFLATEGDHLQLSEEWFYAHIIPFLG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 palmitoyl-protein thioesterase 1
别名 CLN1, EC:3.1.2.22, INCL, Palmitoyl-protein hydrolase 1, Palmitoyl-protein thioesterase 1
计算分子量 34 kDa
观测分子量32-34 kDa
GenBank蛋白编号BC008426
基因名称 PPT1
Gene ID (NCBI) 5538
RRIDAB_2923599
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50897
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The defective gene behind the INCL(infantile neuronal ceroid lipofuscinoses) disease, CLN1, encodes for palmitoyl protein thioesterase 1 (PPT1). It consists of 306 amino acids, including a signal sequence of 26 amino acids and three N-linked glycosylation sites. This protein exists with a molecular mass of 32 kDa, 34 kDa,36 kDa, and 38 kDa. The enzyme is transported into lysosomes of non-neuronal cells by the mannose 6-phosphate receptor (M6PR) mediated pathway and a significant amount of native PPT1 resides in a complex rather than in a monomeric form(PMID:17565660).

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