验证数据展示
经过测试的应用
| Positive WB detected in | U2OS cells, HeLa cells, MDA-MB-453 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32194-1-AP targets PPPDE2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | PPPDE2 fusion protein Ag37655 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | PPPDE peptidase domain containing 2 |
| 别名 | FAM152B, EC:3.4.-.-, EC:3.1.2.22, Desumoylating isopeptidase 1, DeSI-1 |
| 计算分子量 | 168 aa, 18 kDa |
| 观测分子量 | 17 kDa |
| GenBank蛋白编号 | BC112179 |
| 基因名称 | PPPDE2 |
| Gene ID (NCBI) | 27351 |
| RRID | AB_3670220 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q6ICB0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PPPDE2 belongs to N1pC/P60 superfamily of papain-like enzymes, which comprise an extremely diverse group of proteins originating from archaea, bacteria, bacteriophages, viruses, and eukaryotes with predicted protease, amidase, transglutaminases, or acyltransferase activity but often uncharacterized function (PMID: 12620121). PPPDE2 exhibits palmitoyl protein thioesterase (S-depalmitoylation) activity towards synthetic substrates 4-methylumbelliferyl-6-S-palmitoyl-beta-D-glucopyranoside and S-depalmitoylation probe 5 (DPP-5) (PMID:35427157).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPPDE2 antibody 32194-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |