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验证数据展示

  • WB analysis using 87155-1-RR

    WB analysis using 87155-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87155-1-RR (PPP6C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 87155-1-RR

    IF Staining of HEK-293 using 87155-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using PPP6C antibody (87155-1-RR, Clone: 252251E9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 87155-1-RR

    Affinity of 87155-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87155-1-RR against Human PPP6C were performed. The affinity constant is 49.7 nM.

PPP6C Recombinant monoclonal antibody

PPP6C Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 87155-1-RR

产品说明书

CloneNo. 252251E9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

EC:3.1.3.16, PP6C, PPP6, Serine/threonine-protein phosphatase 6 catalytic subunit, Serine/threonine-protein phosphatase 6 catalytic subunit, N-terminally processed

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293T cells, Jurkat cells, NIH/3T3 cells, mouse testis tissue, rat testis tissue
Planning an IHC experiment? We recommend our IHCeasy PPP6C Ready-To-Use IHC Kit. PPP6C primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87155-1-RR targets PPP6C in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8554

Product name: Recombinant human PPP6C protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-305 aa of BC006990

Sequence: MAPLDLDKYVEIARLCKYLPENDLKRLCDYVCDLLLEESNVQPVSTPVTVCGDIHGQFYDLCELFRTGGQVPDTNYIFMGDFVDRGYYSLETFTYLLALKAKWPDRITLLRGNHESRQITQVYGFYDECQTKYGNANAWRYCTKVFDMLTVAALIDEQILCVHGGLSPDIKTLDQIRTIERNQEIPHKGAFCDLVWSDPEDVDTWAISPRGAGWLFGAKVTNEFVHINNLKLICRAHQLVHEGYKFMFDEKLVTVWSAPNYCYRCGNIASIMVFKDVNTREPKLFRAVPDSERVIPPRTTTPYFL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein phosphatase 6, catalytic subunit
别名 EC:3.1.3.16, PP6C, PPP6, Serine/threonine-protein phosphatase 6 catalytic subunit, Serine/threonine-protein phosphatase 6 catalytic subunit, N-terminally processed
计算分子量 305 aa, 35 kDa
观测分子量 30-35 kDa
GenBank蛋白编号BC006990
基因名称 PPP6C
Gene ID (NCBI) 5537
RRIDAB_3745376
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO00743
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP6C (Protein Phosphatase 6 Catalytic Subunit or simply PP6) is a highly conserved serine/threonine phosphatase belonging to the PP2A-like subfamily. This enzyme complex plays critical roles in a wide array of fundamental cellular processes, including cell cycle progression, DNA damage response, NF-κB signaling, and microtubule organization. PPP6C mutations are found across multiple cancer types but are most common in melanoma and other skin cancers (PMID: 33789117).

实验方案

Product Specific Protocols
IF protocol for PPP6C antibody 87155-1-RRDownload protocol
WB protocol for PPP6C antibody 87155-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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