验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14005-1-AP targets PPP3R2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag5097 Product name: Recombinant human PPP3R2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-173 aa of BC030595 Sequence: MSTMGNEASYPAEMCSHFDNDEIKRLGRRFKKLDLDKSGSLSVEEFMSLPELRHNPLVRRVIDVFDTDGDGEVDFKEFILGTSQFSVKGDEEQKLRFAFSIYDMDKDGYISNGELFQVLKMMVGNNLTDWQLQQLVDKTIIILDKDGDGKISFEEFSAVVRDLEIHKKLVLIV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 3 (formerly 2B), regulatory subunit B, beta isoform |
| 别名 | Calcineurin B like protein, Calcineurin BII, Calcineurin B-like protein, Calcineurin subunit B type 2, CBLP |
| 计算分子量 | 20 kDa |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | BC030595 |
| 基因名称 | PPP3R2 |
| Gene ID (NCBI) | 5535 |
| RRID | AB_2168590 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96LZ3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PPP3R2 (protein phosphatase 3 regulatory subunit B, beta) encodes the regulatory subunit B type 2 of calcineurin, a calcium/calmodulin-dependent serine/threonine protein phosphatase. As a member of the calcineurin B subunit family, PPP3R2 contains EF-hand calcium-binding domains and confers calcium sensitivity to the phosphatase complex. Unlike the ubiquitously expressed PPP3R1, PPP3R2 is expressed almost exclusively in the testis, where it assembles with the catalytic subunit PPP3CC to form a testis-enriched "sperm calcineurin". In mature spermatozoa, this complex is localized to the mitochondrial sheath of the midpiece and the flagellum, and it is essential for proper sperm motility, structural integrity of the midpiece, and male fertility.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PPP3R2 antibody 14005-1-AP | Download protocol |
| IP protocol for PPP3R2 antibody 14005-1-AP | Download protocol |
| WB protocol for PPP3R2 antibody 14005-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis miR-146a facilitates osteoarthritis by regulating cartilage homeostasis via targeting Camk2d and Ppp3r2. | ||
Proc Natl Acad Sci U S A SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. | ||
Eur J Pharmacol Stachydrine hydrochloride suppresses phenylephrine-induced pathological cardiac hypertrophy by inhibiting the calcineurin/nuclear factor of activated T-cell signalling pathway. | ||
FASEB J Roles of glycogen synthase kinase 3 alpha and calcineurin in regulating the ability of sperm to fertilize eggs. |
