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验证数据展示

  • WB analysis of mouse testis using 19653-1-AP

    WB analysis of mouse testis using 19653-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 19653-1-AP (PPP3CC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 19653-1-AP

    IHC staining of human stomach using 19653-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 19653-1-AP (PPP3CC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PPP3CC Polyclonal antibody

PPP3CC Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19653-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

CNA3, CAM-PRP catalytic subunit, CAM PRP catalytic subunit, CALNA3, Calmodulin-dependent calcineurin A subunit gamma isoform

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, human testis tissue, Jurkat cells, MCF-7 cells, mouse brain tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

19653-1-AP targets PPP3CC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 PPP3CC fusion protein Ag7619 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 3 (formerly 2B), catalytic subunit, gamma isoform
别名 CNA3, CAM-PRP catalytic subunit, CAM PRP catalytic subunit, CALNA3, Calmodulin-dependent calcineurin A subunit gamma isoform
计算分子量 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC004864
基因名称 PPP3CC
Gene ID (NCBI) 5533
RRIDAB_10695630
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48454
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPP3CC antibody 19653-1-APDownload protocol
IHC protocol for PPP3CC antibody 19653-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

FASEB J

Roles of glycogen synthase kinase 3 alpha and calcineurin in regulating the ability of sperm to fertilize eggs.

Authors - Souvik Dey
View Article
humanWB

BMC Complement Med Ther

Shikonin reactivates TSGs GADD45B and PPP3CC to block NSCLC cell proliferation and migration through JNK/P38/MAPK signaling pathways

Authors - Yujia Zhao
View Article