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验证数据展示

  • WB analysis of mouse testis using 19653-1-AP

    WB analysis of mouse testis using 19653-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 19653-1-AP (PPP3CC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 19653-1-AP

    IHC staining of human stomach using 19653-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 19653-1-AP (PPP3CC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PPP3CC Polyclonal antibody

PPP3CC Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19653-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

CNA3, CAM-PRP catalytic subunit, CAM PRP catalytic subunit, CALNA3, Calmodulin-dependent calcineurin A subunit gamma isoform

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, human testis tissue, Jurkat cells, MCF-7 cells, mouse brain tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

19653-1-AP targets PPP3CC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原

CatNo: Ag7619

Product name: Recombinant human PPP3CC protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 153-512 aa of BC004864

Sequence: TDYFTFKQECRIKYSEQVYDACMETFDCLPLAALLNQQFLCVHGGMSPEITSLDDIRKLDRFTEPPAFGPVCDLLWSDPSEDYGNEKTLEHYTHNTVRGCSYFYSYPAVCEFLQNNNLLSIIRAHEAQDAGYRMYRKSQATGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELISDDEAEGSTTVRKEIIRNKIRAIGKMARVFSILRQESESVLTLKGLTPTGTLPLGVLSGGKQTIETATVEAVEAREAIRGFSLQHKIRSFEEARGLDRINERMPPRKDSIHAGGPMKSVTSAHSHAAHRSDQGKKAHS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 3 (formerly 2B), catalytic subunit, gamma isoform
别名 CNA3, CAM-PRP catalytic subunit, CAM PRP catalytic subunit, CALNA3, Calmodulin-dependent calcineurin A subunit gamma isoform
计算分子量 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC004864
基因名称 PPP3CC
Gene ID (NCBI) 5533
RRIDAB_10695630
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48454
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPP3CC antibody 19653-1-APDownload protocol
IHC protocol for PPP3CC antibody 19653-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

FASEB J

Roles of glycogen synthase kinase 3 alpha and calcineurin in regulating the ability of sperm to fertilize eggs.

Authors - Souvik Dey
View Article
humanWB

BMC Complement Med Ther

Shikonin reactivates TSGs GADD45B and PPP3CC to block NSCLC cell proliferation and migration through JNK/P38/MAPK signaling pathways

Authors - Yujia Zhao
View Article