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PPP2R3C Polyclonal antibody

PPP2R3C Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 24877-1-AP

Print datasheet

Synonyms

C14orf10, G4 1, G5pr, PPP2R3C

验证数据展示

  • WB analysis of mouse brain using 24877-1-AP

    WB analysis of mouse brain using 24877-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24877-1-AP (PPP2R3C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 24877-1-AP

    IHC staining of human brain using 24877-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24877-1-AP (PPP2R3C Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 24877-1-AP

    IHC staining of human brain using 24877-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24877-1-AP (PPP2R3C Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman brain tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24877-1-AP is PPP2R3C Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen PPP2R3C fusion protein Ag19282 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 2 (formerly 2A), regulatory subunit B'', gamma
Synonyms C14orf10, G4 1, G5pr, PPP2R3C
Calculated Molecular Weight 453 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC063438
Gene Symbol PPP2R3C
Gene ID (NCBI) 55012
RRIDAB_2879773
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ969Q6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP2R3C(Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit gamma) is also named as C14orf10, and G5PR. It may regulate MCM3AP phosphorylation through phosphatase recruitment and play a role in the activation-induced cell death of B-cells. It has 2 isoforms produced by alternative splicing with the molecular weight of 53 kDa and 40 kDa.

实验方案

Product Specific Protocols
WB protocol for PPP2R3C antibody 24877-1-APDownload protocol
IHC protocol for PPP2R3C antibody 24877-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols