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PPP2R2A Polyclonal antibody

PPP2R2A Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16569-1-AP

Print datasheet

Synonyms

B55 ALPHA, B55A, PP2A subunit B isoform alpha, PPP2R2A, PR52A, PR55A

验证数据展示

  • WB analysis using 16569-1-AP

    WB analysis using 16569-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16569-1-AP (PPP2R2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 16569-1-AP

    IHC staining of mouse brain using 16569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16569-1-AP (PPP2R2A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 16569-1-AP

    IHC staining of mouse brain using 16569-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16569-1-AP (PPP2R2A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 16569-1-AP

    IF Staining of A431 using 16569-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PPP2R2A antibody (16569-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inK-562 cells, MCF-7 cells, C6 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

16569-1-AP targets PPP2R2A in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen PPP2R2A fusion protein Ag9851 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 2 (formerly 2A), regulatory subunit B, alpha isoform
Synonyms B55 ALPHA, B55A, PP2A subunit B isoform alpha, PPP2R2A, PR52A, PR55A
Calculated Molecular Weight 477 aa, 52 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC041071
Gene Symbol PPP2R2A
Gene ID (NCBI) 5520
RRIDAB_2878280
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP63151
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The serine/threonine protein phosphatase 2A (PP2A) is a master regulator of the complex cellular signaling that occurs during all stages of mammalian development. PPP2R2A, also known as B55A or PR55A, is a regulatory subunit of PP2A expressed in an Akt-dependent manner in epidermis during barrier formation. PPP2R2A participates in the negative control of cell growth and division. PPP2R2A has been proved to be a tumor suppressor that can inhibit the proliferation of a variety of cancer cells, such as prostate cancer cells(PMID: 21872824).

实验方案

Product Specific Protocols
WB protocol for PPP2R2A antibody 16569-1-APDownload protocol
IHC protocol for PPP2R2A antibody 16569-1-APDownload protocol
IF protocol for PPP2R2A antibody 16569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Physiol Biochem

Quantitative Proteomic Analysis of the Metastasis-Inhibitory Mechanism of miR-193a-3p in Non-Small Cell Lung Cancer.

Authors - Wei Deng
View Article
mouseWB

EMBO Rep

CIP2A deficiency promotes depression-like behaviors in mice through inhibition of dendritic arborization

Authors - Wen-Ting Hu
View Article