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PPP2R1B Polyclonal antibody

PPP2R1B Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

IF/ICC, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 12621-1-AP

Print datasheet

Synonyms

PP2A subunit A isoform R1 beta, PPP2R1B, PR65B

验证数据展示

  • WB analysis using 12621-1-AP

    WB analysis using 12621-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12621-1-AP (PPP2R1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 12621-1-AP

    IHC staining of human liver using 12621-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12621-1-AP (PPP2R1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Caco-2 using 12621-1-AP

    IF Staining of Caco-2 using 12621-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using PPP2R1B antibody (12621-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA2780 cells, human kidney tissue, mouse testis tissue, rat testis tissue, HeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCaco-2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12621-1-AP targets PPP2R1B in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IHC, CoIP
Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse
Immunogen PPP2R1B fusion protein Ag3307 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform
Synonyms PP2A subunit A isoform R1 beta, PPP2R1B, PR65B
Calculated Molecular Weight 667 aa, 74 kDa
Observed Molecular Weight 60-74 kDa
GenBank Accession NumberBC027596
Gene Symbol PPP2R1B
Gene ID (NCBI) 5519
RRIDAB_2169941
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30154
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPP2R1B antibody 12621-1-APDownload protocol
IHC protocol for PPP2R1B antibody 12621-1-APDownload protocol
IF protocol for PPP2R1B antibody 12621-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

BRG1 Activates PR65A Transcription to Regulate NO Bioavailability in Vascular Endothelial Cells.

Authors - Baoyu Chen
View Article
mouseWB

EMBO Rep

CIP2A deficiency promotes depression-like behaviors in mice through inhibition of dendritic arborization

Authors - Wen-Ting Hu
View Article
human,mouseWB,IHC,CoIP

Cancer Cell Int

PPP2R1B abolishes colorectal cancer liver metastasis and sensitizes Oxaliplatin by inhibiting MAPK/ERK signaling pathway

Authors - Wei Liu
  • KD Validated
View Article