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验证数据展示

  • IF Staining of MCF-7 using 84155-6-RR

    IF Staining of MCF-7 using 84155-6-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPP2CA antibody (84155-6-RR, Clone: 241112F3 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 84155-6-RR

    FC experiment of HeLa using 84155-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PPP2CA Recombinant antibody (84155-6-RR, Clone:241112F3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 84155-6-RR

    Affinity and Kinetic Characterization of 84155-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84155-6-RR against Human PPP2CA were performed. The affinity constant is 24.4 nM.

PPP2CA Recombinant monoclonal antibody

PPP2CA Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 84155-6-RR

产品说明书

CloneNo. 241112F3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

241112F3, EC:3.1.3.16, PP2A alpha, PP2A-alpha, PP2Ac

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PPP2CA Ready-To-Use IHC Kit. PPP2CA primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84155-6-RR targets PPP2CA in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4294

Product name: Recombinant human PPP2CA protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-309 aa of BC031696

Sequence: MDEKVFTKELDQWIEQLNECKQLSESQVKSLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFRIGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYRERITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTRRTPDYFL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
别名 241112F3, EC:3.1.3.16, PP2A alpha, PP2A-alpha, PP2Ac
计算分子量 309 aa, 36 kDa
GenBank蛋白编号BC031696
基因名称 PPP2CA
Gene ID (NCBI) 5515
RRIDAB_3671720
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP67775
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.

实验方案

Product Specific Protocols
FC protocol for PPP2CA antibody 84155-6-RRDownload protocol
IF protocol for PPP2CA antibody 84155-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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