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验证数据展示

  • WB analysis of PC-3 using 18801-1-AP

    WB analysis of PC-3 using 18801-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18801-1-AP (PPP1R9A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 18801-1-AP

    IF Staining of HepG2 using 18801-1-AP

    Immunofluorescent analysis of HepG2 cells, using PPP1R9A antibody 18801-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

PPP1R9A Polyclonal antibody

PPP1R9A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 18801-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

KIAA1222, Neurabin 1, Neurabin I, NRB1, NRBI, PPP1R9A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells
Positive IF/ICC detected inHepG2 cells, Hela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18801-1-AP targets PPP1R9A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 1, regulatory (inhibitor) subunit 9A
别名 KIAA1222, Neurabin 1, Neurabin I, NRB1, NRBI, PPP1R9A
计算分子量 123 kDa
观测分子量 180 kDa
GenBank蛋白编号BC150636
基因名称 PPP1R9A
Gene ID (NCBI) 55607
RRIDAB_10597849
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9ULJ8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP1R9A, also named as KIAA1222 and NEB1, binds to actin filaments (F-actin) and shows cross-linking activity. PPP1R9A has been found to be concentrated in the synapse of developed neurons and in the lamellipodia of the growth cone during neuron development.PPP1R9A may be involved in neurite formation. PPP1R9A inhibits protein phosphatase 1-alpha activity.

实验方案

Product Specific Protocols
WB protocol for PPP1R9A antibody 18801-1-APDownload protocol
IF protocol for PPP1R9A antibody 18801-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols