验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55389-1-AP targets PPP1R3G in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 1, regulatory (inhibitor) subunit 3G |
| 别名 | Protein phosphatase 1 regulatory subunit 3G |
| 计算分子量 | 38 kDa |
| 观测分子量 | 38-40 kDa |
| GenBank蛋白编号 | NM_001145115 |
| 基因名称 | PPP1R3G |
| Gene ID (NCBI) | 648791 |
| RRID | AB_3743208 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | B7ZBB8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PPP1R3G (protein phosphatase 1 regulatory subunit 3G) is an important member of phosphatase 1 inhibitor -3 family, which is mainly expressed in liver and muscle and participates in glycogen synthesis and metabolism, insulin signaling pathway and lipid metabolism. PPP1R3G is the direct target of insulin -AKT signaling pathway. Insulin phosphorylates Ser79 site of PPP1R3G through AKT, which promotes its binding with GS, so that GS is dephosphorylated by PP1, thus enhancing glycogen synthesis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP1R3G antibody 55389-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
