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PPP1R3C Polyclonal antibody

PPP1R3C Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29424-1-AP

Print datasheet

Synonyms

PP1 subunit R5, PPP1R3C, PPP1R5, Protein targeting to glycogen, PTG

验证数据展示

  • WB analysis using 29424-1-AP

    WB analysis using 29424-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29424-1-AP (PPP1R3C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 29424-1-AP

    IHC staining of human colon cancer using 29424-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29424-1-AP (PPP1R3C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 29424-1-AP

    IHC staining of human colon cancer using 29424-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29424-1-AP (PPP1R3C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293T cells, mouse cerebellum tissue, rat cerebellum tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29424-1-AP targets PPP1R3C in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen PPP1R3C fusion protein Ag30280 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 3C
Synonyms PP1 subunit R5, PPP1R3C, PPP1R5, Protein targeting to glycogen, PTG
Calculated Molecular Weight 317 aa, 36 kDa
GenBank Accession NumberBC012625
Gene Symbol PPP1R3C
Gene ID (NCBI) 5507
RRIDAB_2918304
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UQK1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

• Protein phosphatase 1 regulatory subunit 3C (PPP1R3C) is an enzyme that binds to protein phosphatase-1 (PP1) as a regulator. The central role of PPP1R3C is associated with glycogenesis and the overexpression of this gene increases glycogen storage. The cellular glycogen level is suppressed when PPP1R3C is knocked-down. PPP1R3C induces glycogen accumulation via HIF1 α during hypoxia and it is hypermethylated in melanoma (PMID: 25846879). Overexpression of PPP1R3C increased hepatic gluconeogenesis in vitro and in vivo while knockdown of PPP1R3C showed an opposite result (PMID: 31181215).

实验方案

Product Specific Protocols
WB protocol for PPP1R3C antibody 29424-1-APDownload protocol
IHC protocol for PPP1R3C antibody 29424-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols