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验证数据展示

  • WB analysis using 22495-1-AP

    WB analysis using 22495-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22495-1-AP (PPP1R3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 22495-1-AP

    IF Staining of THP-1 using 22495-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using PPP1R3A antibody (22495-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PPP1R3A Polyclonal antibody

PPP1R3A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 22495-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

RG1, Protein phosphatase type-1 glycogen targeting subunit, Protein phosphatase 1 glycogen-associated regulatory subunit, PPP1R3, PP1G

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, THP-1 cells
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22495-1-AP targets PPP1R3A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18013

Product name: Recombinant human PPP1R3A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 773-1122 aa of BC126453

Sequence: FDPHEGRNDDSHYTLCQRDTVGVIYDNDFEKESRLGICNVRVDEMEKEETMSMYNPRKTHDREKCGTGNITSVEESSWVITEYQKATSKLDLQLGMLPTDKTVFSENRDHRQVQELSKKTDSDAIVHSAFNSDTNRAPQNSSPFSKHHTEISVSTNEQAIAVENAVTTMASQPISTKSENICNSTREIQGIEKHPYPESKPEEVSRSSGIVTSGSRKERCIGQIFQTEEYSVEKSLGPMILINKPLENMEEARHENEGLVSSGQSLYTSGEKESDSSASTSLPVEESQAQGNESLFSKYTNSKIPYFLLFLIFLITVYHYDLMIGLTFYVLSLSWLSWEEGRQKESVKKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 1, regulatory (inhibitor) subunit 3A
别名 RG1, Protein phosphatase type-1 glycogen targeting subunit, Protein phosphatase 1 glycogen-associated regulatory subunit, PPP1R3, PP1G
计算分子量 1122 aa, 126 kDa
观测分子量110-130 kDa
GenBank蛋白编号BC126453
基因名称 PPP1R3A
Gene ID (NCBI) 5506
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ16821
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP1R3A (Protein Phosphatase 1 Regulatory Subunit 3A), also known as Protein Targeting to Glycogen (PTG), is a regulatory subunit of protein phosphatase 1 (PP1), a major serine/threonine phosphatase involved in metabolic regulation. It plays a crucial role in glycogen metabolism by targeting PP1 to glycogen particles, facilitating glycogen synthesis and breakdown. PPP1R3A is involved in muscle glycogen regulation (PMID: 24264575).

实验方案

Product Specific Protocols
IF protocol for PPP1R3A antibody 22495-1-APDownload protocol
WB protocol for PPP1R3A antibody 22495-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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