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验证数据展示

  • WB analysis using 27976-1-AP (same clone as 27976-1-PBS)

    WB analysis using 27976-1-AP (same clone as 27976-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27976-1-AP (C7orf16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27976-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 27976-1-AP (same clone as 27976-1-PBS)

    IF Staining of U2OS using 27976-1-AP (same clone as 27976-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PPP1R17 antibody (27976-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27976-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 27976-1-AP (same clone as 27976-1-PBS)

    IF Staining of U2OS using 27976-1-AP (same clone as 27976-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PPP1R17 antibody (27976-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27976-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 27976-1-AP (same clone as 27976-1-PBS)

    IHC staining of mouse cerebellum using 27976-1-AP (same clone as 27976-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27976-1-AP (PPP1R17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27976-1-PBS in a different storage buffer formulation.

PPP1R17 Polyclonal antibody, PBS Only

PPP1R17 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 27976-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

C7orf16, G substrate, GSBS, G-substrate, Protein phosphatase 1 regulatory subunit 17

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27976-1-PBS targets PPP1R17 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag15187

Product name: Recombinant human C7orf16 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-155 aa of BC028094

Sequence: MMSTEQMQPLEVSEDRLDKLDPRCSHLDDLSDQFIKDCDLKKKPRKGKNVQATLNVESDQKKPRRKDTPALHIPPFIPGVFSEHLIKRYDVQERHPKGKMIPVLHNTDLEQKKPRRKDTPALHMSPFAAGVTLLRDERPKAIVEDDEKDGDKIAI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 7 open reading frame 16
别名 C7orf16, G substrate, GSBS, G-substrate, Protein phosphatase 1 regulatory subunit 17
计算分子量 155 aa, 18 kDa
观测分子量 20-30 kDa
GenBank蛋白编号BC028094
基因名称 PPP1R17
Gene ID (NCBI) 10842
RRIDAB_3086018
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO96001
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPP1R17, an endogenous substrate for cGMP-dependent protein kinase, exists almost exclusively in cerebellar Purkinje cells, where it is possibly involved in the induction of long-term depression (PMID: 10051666). PPP1R17 inhibits phosphatase activities of protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A) complexes. Allelic variants increase susceptibility to hypercholesterolemia (PMID: 12955585).

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