验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25357-1-AP targets PPP1R16B in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat, pig |
| 免疫原 | PPP1R16B fusion protein Ag19269 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 1, regulatory (inhibitor) subunit 16B |
| 别名 | TIMAP, ANKRD4 |
| 计算分子量 | 567 aa, 64 kDa |
| 观测分子量 | 60 kDa, 70 kDa |
| GenBank蛋白编号 | BC131801 |
| 基因名称 | PPP1R16B |
| Gene ID (NCBI) | 26051 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96T49 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PPP1R16B (Protein Phosphatase 1 Regulatory Subunit 16B), also known as TIMAP or ANKRD4, is a membrane-associated protein that acts as a regulator of protein phosphatase 1 (PP1). It contains five ankyrin repeats, a PP1-interacting domain, and a C-terminal CAAX box domain. The synthesis of PPP1R16B is inhibited by transforming growth factor-beta-1 (TGF-β-1), and it is predominantly expressed in endothelial cells. It has 2 isoforms with the molecular mass of 59 and 64 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP1R16B antibody 25357-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |