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0
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验证数据展示

  • WB analysis using 83016-6-RR (same clone as 83016-6-PBS)

    WB analysis using 83016-6-RR (same clone as 83016-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83016-6-RR (PPP1R15B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83016-6-PBS in a different storage buffer formulation.

  • WB analysis of SGC-7901 using 83016-6-RR (same clone as 83016-6-PBS)

    WB analysis of SGC-7901 using 83016-6-RR (same clone as 83016-6-PBS)

    WB result of PPP1R15B antibody (83016-6-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPP1R15B transfected SGC-7901 cells. This data was developed using the same antibody clone with 83016-6-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83016-6-RR (same clone as 83016-6-PBS)

    IF Staining of HepG2 using 83016-6-RR (same clone as 83016-6-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PPP1R15B antibody (83016-6-RR, Clone: 240052G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83016-6-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 83016-6-RR (same clone as 83016-6-PBS)

    FC experiment of U2OS using 83016-6-RR (same clone as 83016-6-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug PPP1R15B Recombinant antibody (83016-6-RR, Clone:240052G5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83016-6-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PPP1R15B Recombinant antibody, PBS Only

PPP1R15B Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83016-6-PBS

产品说明书

CloneNo. 240052G5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

PPP1R15B;CReP, 240052G5, CREP, FLJ14744

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83016-6-PBS targets PPP1R15B in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 PPP1R15B fusion protein Ag6213 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein phosphatase 1, regulatory (inhibitor) subunit 15B
别名 PPP1R15B;CReP, 240052G5, CREP, FLJ14744
计算分子量 79 kDa
观测分子量100-110 kDa
GenBank蛋白编号BC065280
基因名称 PPP1R15B
Gene ID (NCBI) 84919
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ5SWA1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein phosphatase 1 regulatory subunit 15B(PPP1R15B) is a member of PPP1R15 family, it forms a complex with protein phosphatase1(PP1) and NCK1/2, maintaining low levels of EIF2S1 phosphorylation in unstressed cells, thus is also named constitutive repressor of eIF2alpha phosphorylation (CReP). PPP1R15B is assoicated in endoplasmic reticulum (ER) stress response resulting to a role in translation regulation. Knock-down of PPP1R15B strongly protected mammalian cells against oxidative stress, peroxynitrite stress, and more modestly against accumulation of malfolded proteins in the ER. Recently, PPP1R15B was found to associate with cell membranes and regulate membrane traffic in a PP1c-independent manner, suggesting a novel link between translation and traffic. MW of PPP1R15B is 80 kDa. Observed MW of PPP1R15B is from 100-110 kDa maybe due to phosphorylation (PMID: 28492545).