验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, mouse liver tissue, human heart tissue, mouse skeletal muscle tissue, U2OS cells, A549 cells, PC-3 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
18476-1-AP targets PPP1R14B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | PPP1R14B fusion protein Ag13373 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 1, regulatory (inhibitor) subunit 14B |
| 别名 | SOM172, PNG, PLCB3N, Phospholipase C-beta-3 neighbouring gene protein, PHI 1 |
| 计算分子量 | 147 aa, 16 kDa |
| 观测分子量 | 23 kDa |
| GenBank蛋白编号 | BC014522 |
| 基因名称 | PPP1R14B |
| Gene ID (NCBI) | 26472 |
| RRID | AB_10598019 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96C90 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP1R14B antibody 18476-1-AP | Download protocol |
| IF protocol for PPP1R14B antibody 18476-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Immunity Dysregulated Expression of the Nuclear Exosome Targeting Complex Component Rbm7 in Nonhematopoietic Cells Licenses the Development of Fibrosis. | ||
J Cell Mol Med PPP1R14B is a diagnostic prognostic marker in patients with uterine corpus endometrial carcinoma | ||
Biochim Biophys Acta Mol Basis Dis Lysine lactylation-based insight to understanding the characterization of cervical cancer |
