验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, PC-3 cells, C6 cells |
Planning an IHC experiment? We recommend our IHCeasy PPP1R13L Ready-To-Use IHC Kit. PPP1R13L primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86044-1-RR targets PPP1R13L in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | PPP1R13L fusion protein Ag13273 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 1, regulatory (inhibitor) subunit 13 like |
| 别名 | IASPP, Inhibitor of ASPP protein, NFkB-interacting protein 1, NKIP1, PPP1R13B like protein |
| 计算分子量 | 89 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC064913 |
| 基因名称 | PPP1R13L |
| Gene ID (NCBI) | 10848 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8WUF5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Inhibitor of apoptosis-stimulating protein of p53 (iASPP), encoded by PPP1R13L gene, is often overexpressed in human cancers. The ASPP family includes three members, namely ASPP1, ASPP2, and iASPP, which are specific regulators of p53-, p63-, and p73-mediated apoptosis. ASPP1 and ASPP2 enhance the apoptotic function of p53, whereas iASPP specifically inhibits p53-mediated apoptosis. Overexpression of iASPP is associated with resistance to cisplatin-induced apoptosis and rediation therapy. iASPP plays a pivotal role in regulating cancer cell proliferation and tumor progression.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PPP1R13L antibody 86044-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |