验证数据展示
经过测试的应用
| Positive WB detected in | human heart tissue, HEK-293 cells, mouse testis tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy PPP1R13L Ready-To-Use IHC Kit. PPP1R13L primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
51141-1-AP targets PPP1R13L in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag0635 Product name: Recombinant human PPP1R13L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-240 aa of BC032298 Sequence: MDSEAFQSARDFLDMNFQSLAMKHMDLKQMELDTAAAKVDELTKQLESLWSDSPAPPGPQAGPPSRPPRYSSSSIPEPFGSRGSPRKAATDGADTPFGRSESAPTLHPYSPLSPKGRPSSPRTPLYLQPDAYGSLDRATSPRPRAFDGAGSSLGRAPSPRPGPGPLRQQGPPTPFDFLGRAGSPRGSPLAEGPQAFFPERGPSPRPPATAYDAPASAFGSSLLGSGGSAFAPPLRAQDDL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 1, regulatory (inhibitor) subunit 13 like |
| 别名 | IASPP, Inhibitor of ASPP protein, NFkB interacting protein 1, NKIP1, PPP1R13B like protein, PPP1R13BL, PPP1R13L, Protein iASPP, RAI, RelA associated inhibitor |
| 计算分子量 | 45 kDa, 89 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC032298 |
| 基因名称 | PPP1R13L |
| Gene ID (NCBI) | 10848 |
| RRID | AB_2268619 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WUF5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PPP1R13L antibody 51141-1-AP | Download protocol |
| WB protocol for PPP1R13L antibody 51141-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Med Exome-wide evaluation of rare coding variants using electronic health records identifies new gene-phenotype associations. | ||
Adv Sci (Weinh) Chitosan-Gelatin-EGCG Nanoparticle-Meditated LncRNA TMEM44-AS1 Silencing to Activate the P53 Signaling Pathway for the Synergistic Reversal of 5-FU Resistance in Gastric Cancer
| ||
