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验证数据展示

  • WB analysis using 87808-1-RR (same clone as 87808-1-PBS)

    WB analysis using 87808-1-RR (same clone as 87808-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87808-1-RR (PPP1R12B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87808-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 87808-1-RR (same clone as 87808-1-PBS)

    IF Staining of NIH/3T3 using 87808-1-RR (same clone as 87808-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using PPP1R12B antibody (87808-1-RR, Clone: 253416D1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87808-1-PBS in a different storage buffer formulation.

  • Affinity of 87808-1-RR

    Affinity of 87808-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87808-1-RR against Human PPP1R12B were performed. The affinity constant is 0.905 nM.

PPP1R12B Recombinant monoclonal antibody, PBS Only

PPP1R12B Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87808-1-PBS

产品说明书

CloneNo. 253416D1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

Myosin phosphatase-targeting subunit 2, MYPT2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87808-1-PBS targets PPP1R12B in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4185

Product name: Recombinant human PPP1R12B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-386 aa of BC034430

Sequence: MAELEHLGGKRAESARMRRAEQLRRWRGSLTEQEPAERRGAGRQPLTRRGSPRVRFEDGAVFLAACSSGDTDEVRKLLARGADINTVNVDGLTALHQACIDENLDMVKFLVENRANVNQQDNEGWTPLHAAASCGYLNIAEYFINHGASVGIVNSEGEVPSDLAEEPAMKDLLLEQVKKQGVDLEQSRKEEEQQMLQDARQWLNSGKIEDVRQARSGATALHVAAAKGYSEVLRLLIQAGYELNVQDYDGWTPLHAAAHWGVKEACSILAEALCDMDIRNKLGQTPFDVADEGLVEHLELLQKKQNVLRSEKETRNKLIESDLNSKIQSGFFKNKEKMLYEEETPKSQEMEEENKESSSSSSEEEEGEDEASESETEKEAVLFWPF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein phosphatase 1, regulatory (inhibitor) subunit 12B
别名 Myosin phosphatase-targeting subunit 2, MYPT2
计算分子量 982 aa, 110 kDa
观测分子量 110-130 kDa
GenBank蛋白编号BC034430
基因名称 PPP1R12B
Gene ID (NCBI) 4660
RRIDAB_3745761
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO60237
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPP1R12B (MYPT2) is a critical regulatory subunit of protein phosphatase 1, primarily functioning in muscle tissues to control myosin phosphatase activity and calcium sensitivity of contraction. Its tissue-specific expression, particularly enrichment in heart and skeletal muscle, along with multiple alternatively spliced isoforms, enables specialized roles in different cellular contexts. The protein's ankyrin repeats and C-terminal kinase-interacting domain facilitate its function as a molecular scaffold that directs PP1 catalytic activity toward specific substrates.

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