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PPP1R12B Polyclonal antibody

PPP1R12B Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 13366-1-AP

Print datasheet

Synonyms

MYPT2, PPP1R12B

验证数据展示

  • WB analysis using 13366-1-AP

    WB analysis using 13366-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13366-1-AP (PPP1R12B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 13366-1-AP

    IHC staining of mouse heart using 13366-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13366-1-AP (PPP1R12B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 13366-1-AP

    IHC staining of mouse heart using 13366-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13366-1-AP (PPP1R12B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of NIH/3T3 using 13366-1-AP

    IF Staining of NIH/3T3 using 13366-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 13366-1-AP (PPP1R12B antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13366-1-AP targets PPP1R12B in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Immunogen PPP1R12B fusion protein Ag4185 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 12B
Synonyms MYPT2, PPP1R12B
Calculated Molecular Weight 982 aa, 110 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC034430
Gene Symbol PPP1R12B
Gene ID (NCBI) 4660
RRIDAB_2168445
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60237
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPP1R12B antibody 13366-1-APDownload protocol
IHC protocol for PPP1R12B antibody 13366-1-APDownload protocol
IF protocol for PPP1R12B antibody 13366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,IF

EBioMedicine

Epistatic evidence for gender-dependant slow neurotransmission signalling in substance use disorders: PPP1R12B versus PPP1R1B.

Authors - Kefu Liu
View Article
mouseWB

Skelet Muscle

Novel γ-sarcoglycan interactors in murine muscle membranes.

Authors - Tara C Smith
View Article
mouseIF

J Biol Chem

The MYPT2-regulated striated muscle-specific myosin light chain phosphatase limits cardiac myosin phosphorylation in vivo

Authors - Eunyoung Lee
View Article