验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IP | See 1 publications below |
产品信息
24450-1-AP targets PPP1R10 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag20091 Product name: Recombinant human PPP1R10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-318 aa of BC150310 Sequence: MGSGPIDPKELLKGLDSFLNRDGEVKSVDGISKIFSLMKEARKMVSRCTYLNILLQTRSPEILVKFIDVGGYKLLNNWLTYSKTTNNIPLLQQILLTLQHLPLTVDHLKQNNTAKLVKQLSKSSEDEELRKLASVLVSDWMAVIRSQSSTQPAEKDKKKRKDEGKSRTTLPERPLTEVKAETRAEEAPEKKREKPKSLRTTAPSHAKFRSTGLELETPSLVPVKKNASTVVVSDKYNLKPIPLKRQSNVAAPGDATPPAEKKYKPLNTTPNATKEIKVKIIPPQPMEGLGFLDALNSAPVPGIKIKKKKKVLSPTAAK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein phosphatase 1, regulatory (inhibitor) subunit 10 |
| 别名 | CAT53, FB19, p99, PNUTS, PP1 binding protein of 114 kDa, PP1R10, PPP1R10, Protein FB19 |
| 计算分子量 | 940 aa, 99 kDa |
| 观测分子量 | 99 kDa |
| GenBank蛋白编号 | BC150310 |
| 基因名称 | PPP1R10 |
| Gene ID (NCBI) | 5514 |
| RRID | AB_2879554 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96QC0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PPP1R10 antibody 24450-1-AP | Download protocol |
| WB protocol for PPP1R10 antibody 24450-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol A regulated PNUTS mRNA to lncRNA splice switch mediates EMT and tumour progression. | ||
J Exp Clin Cancer Res Radiotherapy resistance driven by Asparagine endopeptidase through ATR pathway modulation in breast cancer |
