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验证数据展示

  • WB analysis using 55150-1-AP

    WB analysis using 55150-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55150-1-AP (PPP1CC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 55150-1-AP

    IHC staining of human heart using 55150-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 55150-1-AP (PPP1CC antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with PPP1CC antibody included
  • IHC staining of human heart using 55150-1-AP

    IHC staining of human heart using 55150-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 55150-1-AP (PPP1CC antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with PPP1CC antibody included
  • IP experiment of mouse brain using 55150-1-AP

    IP experiment of mouse brain using 55150-1-AP

    IP result of anti-PPP1CC (IP:55150-1-AP, 4ug; Detection:55150-1-AP 1:500) with mouse brain tissue lysate 3440ug.

  • IF Staining of HeLa using 55150-1-AP

    IF Staining of HeLa using 55150-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PPP1CC antibody (55150-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PPP1CC Polyclonal antibody

PPP1CC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 55150-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Serine/threonine-protein phosphatase PP1-gamma catalytic subunit, PP-1G, EC:3.1.3.16

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, L02 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PPP1CC Ready-To-Use IHC Kit. PPP1CC primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

55150-1-AP targets PPP1CC in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 1, catalytic subunit, gamma isoform
别名 Serine/threonine-protein phosphatase PP1-gamma catalytic subunit, PP-1G, EC:3.1.3.16
计算分子量 38 kDa
观测分子量 35 kDa
GenBank蛋白编号NM_002710
基因名称 PPP1CC
Gene ID (NCBI) 5501
RRIDAB_10792814
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP36873
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP1CC, also named as PP-1G, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. It is involved in regulation of ionic conductances and long-term synaptic plasticity. PPP1CC may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. The antibody is specific to PPP1CC.

实验方案

Product Specific Protocols
WB protocol for PPP1CC antibody 55150-1-APDownload protocol
IHC protocol for PPP1CC antibody 55150-1-APDownload protocol
IF protocol for PPP1CC antibody 55150-1-APDownload protocol
IP protocol for PPP1CC antibody 55150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Cell Dev Biol

PERK Signaling Controls Myoblast Differentiation by Regulating MicroRNA Networks.

Authors - Ye-Ya Tan
View Article
mouseWB

J Biol Chem

Hydrogen sulfide modulates eukaryotic translation initiation factor 2α (eIF2α) phosphorylation status in the integrated stress response pathway.

Authors - Vinita Yadav
View Article
humanWB

Cell Death Dis

Genome-wide identification of microRNA targets reveals positive regulation of the Hippo pathway by miR-122 during liver development.

Authors - Yin Zhang
View Article