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PPP1CA Polyclonal antibody

PPP1CA Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 28617-1-AP

Print datasheet

Synonyms

PP 1A, PPP1A, PPP1CA

验证数据展示

  • WB analysis using 28617-1-AP

    WB analysis using 28617-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28617-1-AP (PPP1CA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 28617-1-AP

    IHC staining of human breast cancer using 28617-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28617-1-AP (PPP1CA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 28617-1-AP

    IHC staining of human breast cancer using 28617-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28617-1-AP (PPP1CA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, LNCaP cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28617-1-AP targets PPP1CA in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human
Immunogen PPP1CA fusion protein Ag28451 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 1, catalytic subunit, alpha isoform
Synonyms PP 1A, PPP1A, PPP1CA
Calculated Molecular Weight 330 aa, 38 kDa
Observed Molecular Weight37 kDa
GenBank Accession NumberBC001888
Gene Symbol PPP1CA
Gene ID (NCBI) 5499
RRIDAB_2918182
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62136
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPP1CA antibody 28617-1-APDownload protocol
IHC protocol for PPP1CA antibody 28617-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols