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验证数据展示

  • WB analysis of human lung using 12104-1-AP

    WB analysis of human lung using 12104-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 12104-1-AP (PPM1M antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse lung using 12104-1-AP

    IP experiment of mouse lung using 12104-1-AP

    IP result of anti-PPM1M (IP:12104-1-AP, 4ug; Detection:12104-1-AP 1:300) with mouse lung tissue lysate 4000ug.

PPM1M Polyclonal antibody

PPM1M Polyclonal Antibody for WB, IP, ELISA
Cat No. 12104-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

别名

PP2C eta, PP2CE, PP2Ceta, PPM1E, PPM1M, Protein phosphatase 1M

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman lung tissue
Positive IP detected inmouse lung tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12104-1-AP targets PPM1M in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag2748

Product name: Recombinant human PPM1M protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-247 aa of BC009644

Sequence: MGGCTALVAVSLQGKLYMANAGDSRAILVRRDEIRPLSFEFTPETERQRIQQLAFVYPELLAGEFTRLEFPRRLKGDDLGQKVLFRDHHMSGWSYKRVEKSDLKYPLIHGQGRQARLLGTLAVSRGLGDHQLRVLDTNIQLKPFLLSVPQVTVLDVDQLELQEDDVVVMATDGLWDVLSNEQVAWLVRSFLPGNQEDPHRFSKLAQMLIHSTQGKEDSLTEEGQVSYDDVSVFVIPLHSQGQESSDH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 1M (PP2C domain containing)
别名 PP2C eta, PP2CE, PP2Ceta, PPM1E, PPM1M, Protein phosphatase 1M
计算分子量 270 aa, 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC009644
基因名称 PPM1M
Gene ID (NCBI) 132160
RRIDAB_2170362
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96MI6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IP protocol for PPM1M antibody 12104-1-APDownload protocol
WB protocol for PPM1M antibody 12104-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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